Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection

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dc.contributor.authorSánchez-Torres, Paloma
dc.contributor.authorVilanova, Laura
dc.contributor.authorBallester, Ana-Rosa
dc.contributor.authorLópez-Pérez, M.
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorViñas Almenar, Inmaculada
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorGonzález-Candelas, Luis
dc.contributor.authorTorres Sanchis, Rosario
dc.date.accessioned2019-03-14T12:38:47Z
dc.date.available2019-03-14T12:38:47Z
dc.date.issued2018
dc.description.abstractBlue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 °C. The ΔPeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the ΔPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in ΔPeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction.ca_ES
dc.description.sponsorshipAuthors are grateful to the Spanish Government for its financial support with the projects AGL2008-04828-C03-03, AGL2011-30519-C03-01, AGL2011-30519-C03-02 and AGL2011-30519-CO3-03 from the “Ministerio de Economía y Competitividad” (MINECO, Spain), the CERCA Programme/Generalitat de Catalunya and Generalitat Valenciana (PROMETEOII/2014/027).ca_ES
dc.identifier.doihttps://doi.org/10.1016/j.fm.2017.08.005
dc.identifier.idgrec025938
dc.identifier.issn0740-0020
dc.identifier.urihttp://hdl.handle.net/10459.1/65954
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN//AGL2008-04828-C03-03/ES/DETERMINANTES MOLECULARES DE LA ESPECIFICIDAD DE HUESPED Y VIRULENCIA DE ESPECIES DE PENICILLIUM PATOGENAS DE FRUTOS CITRICOS Y MANZANAS/ca_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN//AGL2011-30519-CO3-01/ES/CARACTERIZACION DE LOS MECANISMOS DE VIRULENCIA Y DEFENSA EN LA INTERACCION HONGO-FRUTO COMO HERRAMIENTA PARA EL CONTROL DE PENICILLIUM PATOGENOS DE CITRICOS Y DE MANZANAS/ca_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN//AGL2011-30519-C03-03/ES/CARACTERIZACION DE LOS MECANISMOS DE VIRULENCIA Y DEFENSA EN LA INTERACCION HONGO-FRUTO COMO HERRAMIENTA PARA EL CONTROL DE PENICILLIUM PATOGENOS DE CITRICOS Y DE MANZANAS/ca_ES
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.fm.2017.08.005ca_ES
dc.relation.ispartofFood Microbiology, 2018, vol. 69, p. 123-135ca_ES
dc.rightscc-by-nc-nd (c) Elsevier, 2017ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAppleca_ES
dc.subjectConidiationca_ES
dc.subjectFruit-fungal interactionca_ES
dc.subjectGene expressionca_ES
dc.subjectTranscription factorca_ES
dc.titleUnravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infectionca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersionca_ES
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