The MAP Kinase Slt2 Is Involved in Vacuolar Function and Actin Remodeling in Saccharomyces cerevisiae Mutants Affected by Endogenous Oxidative Stress

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dc.contributor.authorPujol Carrión, Núria
dc.contributor.authorPetkova, Mima Ivanova
dc.contributor.authorSerrano Endolz, Luis
dc.contributor.authorTorre Ruiz, M. A. de la
dc.date.accessioned2017-02-02T11:37:18Z
dc.date.available2017-02-02T11:37:18Z
dc.date.issued2013
dc.description.abstractOxidative stress causes transient actin cytoskeleton depolarization and also provokes vacuole fragmentation in wild-type cells. Under conditions of oxidative stress induced by hydrogen peroxide, the Slt2 protein is required to repolarize the actin cytoskeleton and to promote vacuole fusion. In this study, we show that grx3 grx4 and grx5 mutants are cellular models of endogenous oxidative stress. This stress is the result of alterations in iron homeostasis that lead to impairment of vacuolar function and also to disorganization of the actin cytoskeleton. Slt2 overexpression suppresses defects in vacuolar function and actin cytoskeleton organization in the grx3 grx4 mutant. Slt2 exerts this effect independently of the intracellular levels of reactive oxygen species (ROS) and of iron homeostasis. The deletion of SLT2 in the grx3 grx4 mutant results in synthetic lethality related to vacuolar function with substantial vacuole fragmentation. The observation that both Vps4 and Vps73 (two proteins related to vacuole sorting) suppress vacuole fragmentation and actin depolarization in the grx3 grx4 slt2 triple mutant strengthens the hypothesis that Slt2 plays a role in vacuole homeostasis related to actin dynamics. Here we show that in sod1, grx5, and grx3 grx4 slt2 mutants, all of which are affected by chronic oxidative stress, the overexpression of Slt2 favors vacuole fusion through a mechanism dependent on an active actin cytoskeleton.ca_ES
dc.description.sponsorshipThis research was supported by grants BFU2009-11215 and BFU2012-31407 from the MEC (Spanish Government). Nuria Pujol-Carrion was supported by a contract associated with grant BFU2009-11215, and Mima I. Petkova was supported by a fellowship from the Generalitat de Catalunya (Spain) and a contract funded by grant 2009SGR196 from the Generalitat de Catalunya (Spain).ca_ES
dc.identifier.doihttps://doi.org/10.1128/AEM.01692-13
dc.identifier.idgrec020086
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10459.1/59178
dc.language.isoengca_ES
dc.publisherAmerican Society for Microbiologyca_ES
dc.relationMICINN/PN2008-2011/BFU2009-11215ca_ES
dc.relationMICINN/PN2008-2011/BFU2012-31407ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1128/AEM.01692-13ca_ES
dc.relation.ispartofApplied and Environmental Microbiology, 2013, vol. 79, núm. 20, p. 6459-6471ca_ES
dc.rights(c) American Society for Microbiology, 2013ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.subject.otherBiologia molecularca_ES
dc.subject.otherEstrès oxidatiuca_ES
dc.subject.otherBioquímicaca_ES
dc.titleThe MAP Kinase Slt2 Is Involved in Vacuolar Function and Actin Remodeling in Saccharomyces cerevisiae Mutants Affected by Endogenous Oxidative Stressca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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