Biopreservation of fresh-cut melon using the strain Pseudomonas graminis CPA-7
| dc.contributor.author | Abadias i Sero, Mª Isabel | |
| dc.contributor.author | Altisent Rosell, Rosa | |
| dc.contributor.author | Usall i Rodié, Josep | |
| dc.contributor.author | Torres Sanchis, Rosario | |
| dc.contributor.author | Sousa Oliveira, Márcia Patrícia de | |
| dc.contributor.author | Viñas Almenar, Inmaculada | |
| dc.date.accessioned | 2017-05-19T13:33:41Z | |
| dc.date.embargoEndDate | 2025-01-01 | |
| dc.date.issued | 2014 | |
| dc.date.updated | 2017-05-19T13:33:42Z | |
| dc.description.abstract | The use of biopreservation is a promising technique to ensure microbial safety of fresh-cut produce. The objective of this work was to test the effectiveness of the strain CPA-7 of Pseudomonas graminis against a cocktail of Salmonella spp. and Listeria monocytogenes on fresh-cut melon, and evaluate its effect on its quality during shelf-life when tested in conditions simulating commercial application. Fresh-cut melon was artificially inoculated with Salmonella spp. and L. monocytogenes and with or without the biopreservative strain at different concentrations and stored at 20, 10 and 5 °C. Moreover, the effect of the strain was tested in conditions simulating commercial application. Fresh-cut melon was packaged using passive modified atmosphere (MAP) and AIR conditions and stored at 5 and 10 °C. Quality of fresh-cut melon was evaluated in CPA-7 treated and untreated samples. At laboratory scale trials, P. graminis reduced Salmonella and L. monocytogenes growth on fresh-cut melon stored at 5, 10 and 20 °C. Effectiveness depended on their concentration and on storage temperature. At low pathogen concentration and 20 °C, L. monocytogenes growth was reduced between 2.1 and 5.3 log cfu g−1 units after 2 days of storage and Salmonella growth between 2.0 and 7.3 log cfu g−1 depending on CPA-7 dose. At 10 °C, similar reductions of pathogens were observed after 5 days of storage. In studies simulating commercial conditions, packaging atmosphere and temperature influenced P. graminis effectiveness, with better results in samples packaged under AIR conditions and 10 °C. Reduction of pathogen growth was <1-log unit in fresh-cut melon stored in MAP whilst it was >4-log units in AIR. Soluble solids content, titratable acidity, pH and firmness of fresh-cut melon were not significantly different in CPA-7 treated and untreated (control) melon. In general, lightness, chroma and hue values of fresh-cut melon stored in AIR decreased faster in CPA-7 samples than on control ones. At 5 °C, CPA-7 treated melon was visually scored lower than untreated melon. P. graminis has demonstrated promising results at 10 °C, which is a temperature more compromised for safety. Nevertheless more detailed studies on the modified atmosphere are required because AIR packaging is not recommended due to the rapid loss of quality. | |
| dc.description.sponsorship | Authors are grateful to the Spanish Government for research projects AGL-2009-08506 and AGL-2010-19385. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.doi | https://doi.org/10.1016/j.postharvbio.2014.05.010 | |
| dc.identifier.idgrec | 021320 | |
| dc.identifier.issn | 0925-5214 | |
| dc.identifier.uri | http://hdl.handle.net/10459.1/59659 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation | info:eu-repo/grantAgreement/MICINN//AGL2009-08506/ES/Control De Microbiota Alterante Y De Interes Sanitario En Fruta Minimamente Procesada Mediante El Uso De Diferentes Tecnologias. Control Biologico, Altas Presiones Y Envasado En Atmosfera Modificada/ | |
| dc.relation | info:eu-repo/grantAgreement/MICINN//AGL2010-19385/ES/CONTROL DE LA MICROBIOTA ALTERANTE Y DE INTERES SANITARIO EN FRUTA MINIMAMENTE PROCESADA MEDIANTE CONTROL BIOLOGICO Y ENVASADO EN ATMOSFERAS MODIFICADAS/ | |
| dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.postharvbio.2014.05.010 | |
| dc.relation.ispartof | Postharvest Biology and Technology, 2014, vol. 96, p. 69 -77 | |
| dc.rights | (c) Elsevier, 2014 | |
| dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | |
| dc.subject | Foodborne pathogens | |
| dc.subject | Antagonist | |
| dc.subject | Microbial safety | |
| dc.subject | Salmonella | |
| dc.title | Biopreservation of fresh-cut melon using the strain Pseudomonas graminis CPA-7 | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |