Low Doses of EPO Activate MAP Kinases but Not JAK2–STAT5 in Rat Vascular Smooth Muscle Cells

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2001
Author
Ammarguellat, Fatima
Llovera i Tomàs, Marta
Kelly, Paul A.
Goffin, Vincent
Suggested citation
Ammarguellat, Fatima; Llovera i Tomàs, Marta; Kelly, Paul A.; Goffin, Vincent; . (2001) . Low Doses of EPO Activate MAP Kinases but Not JAK2–STAT5 in Rat Vascular Smooth Muscle Cells. Biochemical and Biophysical Research Communications, 2001, vol. 284, núm. 4, p. 1031-1038. https://doi.org/10.1006/bbrc.2001.5085.
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Abstract
Previous reports have shown a direct effect of erythropoietin (Epo) on vascular smooth muscle cells (VSMCs). Our aim was to assess expression of the Epo receptor (EpoR) on VSMCs and to study the activation of two major signaling cascades activated by Epo, namely JAK2/STAT5 and MAPK pathways. All experiments were performed in parallel using the Eporesponsive UT7 cell line. From semiquantitative RTPCR experiments, VSMCs were estimated to express ;30-fold less EpoR mRNA than UT7 cells. Epo-induced phosphorylation of proteins involved in the EpoR/ JAK2/STAT5 cascade could not be detected in VSMCs, even using pharmacological doses of Epo (250 IU/ml). In contrast, a strong activation of MAP kinase pathway was detected with as low as 10 IU/ml Epo. We suggest that MAPK activation reflects a physiologically relevant effect of Epo on VSMCs that may be correlated to cell proliferation.
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http://hdl.handle.net/10459.1/57003
DOI
https://doi.org/10.1006/bbrc.2001.5085
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Biochemical and Biophysical Research Communications, 2001, vol. 284, núm. 4, p. 1031-1038
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