Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Low Doses of EPO Activate MAP Kinases but Not JAK2–STAT5 in Rat Vascular Smooth Muscle Cells

Thumbnail
View/Open
008512.pdf (382.9Kb)
Sol·licita una còpia
Issue date
2001
Author
Ammarguellat, Fatima
Llovera i Tomàs, Marta
Kelly, Paul A.
Goffin, Vincent
Suggested citation
Ammarguellat, Fatima; Llovera i Tomàs, Marta; Kelly, Paul A.; Goffin, Vincent; . (2001) . Low Doses of EPO Activate MAP Kinases but Not JAK2–STAT5 in Rat Vascular Smooth Muscle Cells. Biochemical and Biophysical Research Communications, 2001, vol. 284, núm. 4, p. 1031-1038. https://doi.org/10.1006/bbrc.2001.5085.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Previous reports have shown a direct effect of erythropoietin (Epo) on vascular smooth muscle cells (VSMCs). Our aim was to assess expression of the Epo receptor (EpoR) on VSMCs and to study the activation of two major signaling cascades activated by Epo, namely JAK2/STAT5 and MAPK pathways. All experiments were performed in parallel using the Eporesponsive UT7 cell line. From semiquantitative RTPCR experiments, VSMCs were estimated to express ;30-fold less EpoR mRNA than UT7 cells. Epo-induced phosphorylation of proteins involved in the EpoR/ JAK2/STAT5 cascade could not be detected in VSMCs, even using pharmacological doses of Epo (250 IU/ml). In contrast, a strong activation of MAP kinase pathway was detected with as low as 10 IU/ml Epo. We suggest that MAPK activation reflects a physiologically relevant effect of Epo on VSMCs that may be correlated to cell proliferation.
URI
http://hdl.handle.net/10459.1/57003
DOI
https://doi.org/10.1006/bbrc.2001.5085
Is part of
Biochemical and Biophysical Research Communications, 2001, vol. 284, núm. 4, p. 1031-1038
European research projects
Collections
  • Articles publicats (Ciències Mèdiques Bàsiques) [559]

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to