Articles publicats (Ciències Mèdiques Bàsiques)

Permanent URI for this collection

Browse

Recent Submissions

Now showing 1 - 5 of 635
  • Item
    Open Access
    Trioxidized cysteine in the aging proteome mimics the structural dynamics and interactome of phosphorylated serine
    (ohn Wiley & Sons, 2023) Sánchez Milán, José Antonio; Fernández-Rhodes, María; Guo, Xue; Mulet, Maria; Cam Ngan, SoFong; Iyappan, Ranjith; Katoueezadeh, Maryam; Sze, Siu Kwan; Serra, Aida; Gallart-Palau, Xavier
    Aging is the primary risk factor for the development of numerous human chronic diseases. On a molecular level, it significantly impacts the regulation of protein modifications, leading to the accumulation of degenerative protein modifications (DPMs) such as aberrant serine phosphorylation (p-Ser) and trioxidized cysteine (t-Cys) within the proteome. The altered p-Ser is linked to abnormal cell signaling, while the accumulation of t-Cys is associated with chronic diseases induced by oxidative stress. Despite this, the potential cross-effects and functional interplay between these two critical molecular factors of aging remain undisclosed. This study analyzes the aging proteome of wild-type C57BL/6NTac mice over 2 years using advanced proteomics and bioinformatics. Our objective is to provide a comprehensive analysis of how t-Cys affects cell signaling and protein structure in the aging process. The results obtained indicate that t-Cys residues accumulate in the aging proteome, interact with p-Ser interacting enzymes, as validated in vitro, and alter their structures similarly to p-Ser. These findings have significant implications for understanding the interplay of oxidative stress and phosphorylation in the aging process. Additionally, they open new venues for further research on the role(s) of these protein modifications in various human chronic diseases and aging, wherein exacerbated oxidation and aberrant phosphorylation are implicated.
  • Item
    Open Access
    Síndrome de Schnitzler y variante en el gen MEFV
    (Elsevier, 2024) Nicolás-Sánchez, F. J.; Aróstegui-Gorospe, J. I.; Tarragona Foradada, Jordi; González-Barranquero, A.
  • Item
    Open Access
    Gastric metaplasia as a precursor of nonconventional dysplasia in inflammatory bowel disease
    (Elsevier, 2024) Musulén, Eva; Gené, Míriam; Cuatrecasas, Miriam; Amat, Irene; Veiga, Jesús Alberto; Fernández Aceñero, María Jesús; Fusté Chimisana, Victòria; Tarragona Foradada, Jordi; Jurado, Ismael; Fernández Victoria, Rebeca; Martínez Ciarpaglini, Carolina; Alenda González, Cristina; Zac, Carlos; Fernández Figueras, María Teresa; Esteller, Manel
    Gastric metaplasia in colonic mucosa with inflammatory bowel disease (IBD) develops as an adaptation mech anism. The association between gastric metaplasia and nonconventional and/or conventional dysplasia as pre cursors of colitis-associated colorectal cancer is unknown. To address this question, we retrospectively reviewed a series of 33 IBD colectomies to identify gastric metaplasia in 76 precursor lesions. We obtained 61 nonconventional and 15 conventional dysplasias. Among nonconventional dysplasia, 31 (50.8 %) were low-grade (LGD), 4 (6.5 %) were high-grade (HGD), 9 (14.8 %) had both LGD and HGD, and 17 (27.9 %) had no dysplasia (ND), while 14 (93 %) conventional dysplasias had LGD, and 1 (7 %) had LGD and HGD. Gastric metaplasia was assessed by concomitant immunoexpression of MUC5AC and loss of CDX2 staining. Expression of a p53-mut pattern was considered as a surrogate for gene mutation, and complete loss of MLH1 staining as presence of MLH1 hypermethylation. In nonconventional dysplasia, MUC5AC immunoexpression decreased as the degree of dysplasia increased, being 78 % in LGD and 39 % in HGD (p = 0.006). CDX2 was lost in epithelial glands with high expression of MUC5AC (p < 0.001). The p53-mut pattern was observed in 77 % HGD, 45 % LGD, and in 6 % with ND (p < 0.001). Neither nonconventional nor conventional dysplasia showed complete loss of MLH1 staining. Gastric metaplasia was also present in mucosa adjacent to nonconventional dysplasia with chronic changes or active inflammation. Our results show that gastric metaplasia appears in IBD-inflamed colon mucosa, it is the substrate of most nonconventional dysplasia and occurs prior to p53 alterations.
  • Item
    Open Access
    Clustering COVID-19 ARDS patients through the first days of ICU admission. An analysis of the CIBERESUCICOVID Cohort
    (BMC, 2024) Ceccato, Adrián; Forne, Carles; Bos, Lieuwe D .; Camprubí-Rimblas, Marta; Areny-Balagueró, Aina; Campaña-Duel, Elena; Quero, Sara; Diaz, Emili; Roca, Oriol; de Gonzalo Calvo, David; Fernández-Barat, Laia; Motos, Anna; Ferrer, Ricard; Riera, Jordi; Lorente, Jose A.; Peñuelas, Oscar; Menendez, Rosario; Amaya-Villar, Rosario; Añón, José M.; Balan-Mariño, Ana; Barberà, Carme; Barberán, José; Blandino-Ortiz, Aaron; Boado, Maria Victoria; Bustamante-Munguira, Elena; Caballero, Jesús; Carbajales, Cristina; Carbonell, Nieves; Catalán-González, Mercedes; Franco, Nieves; Galbán, Cristóbal; Gumucio-Sanguino, Víctor D.; de la Torre, Maria del Carmen; Estella, Ángel; Gallego, Elena; García-Garmendia, José Luis; Garnacho-Montero, José; Gómez, José M.; Huerta, Arturo; Jorge-García, Ruth Noemí; Loza-Vázquez, Ana; Marin-Corral, Judith; Martínez de la Gándara, Amalia; Martin-Delgado, María Cruz; Martínez-Varela, Ignacio; Lopez Messa, Juan; Muñiz-Albaiceta, Guillermo; Nieto, María Teresa; Novo, Mariana Andrea; Peñasco, Yhivian; Pozo-Laderas, Juan Carlos; Pérez-García, Felipe; Ricart, Pilar; Roche-Campo, Ferran; Rodríguez, Alejandro; Sagredo, Victor; Sánchez-Miralles, Angel; Sancho-Chinesta, Susana; Socias, Lorenzo; Solé-Violan, Jordi; Suarez-Sipmann, Fernando; Tamayo-Lomas, Luis; Trenado, José; Úbeda, Alejandro; Valdivia, Luis Jorge; Vidal, Pablo; Bermejo, Jesus; González, Jessica; Barbé Illa, Ferran; Calfee, Carolyn S.; Artigas, Antonio; Torres, Antoni
    Background: Acute respiratory distress syndrome (ARDS) can be classified into sub-phenotypes according to different inflammatory/clinical status. Prognostic enrichment was achieved by grouping patients into hypoinflammatory or hyperinflammatory sub-phenotypes, even though the time of analysis may change the classification according to treatment response or disease evolution. We aimed to evaluate when patients can be clustered in more than 1 group, and how they may change the clustering of patients using data of baseline or day 3, and the prognosis of patients according to their evolution by changing or not the cluster. Methods: Multicenter, observational prospective, and retrospective study of patients admitted due to ARDS related to COVID-19 infection in Spain. Patients were grouped according to a clustering mixed-type data algorithm (k-prototypes) using continuous and categorical readily available variables at baseline and day 3. Results: Of 6205 patients, 3743 (60%) were included in the study. According to silhouette analysis, patients were grouped in two clusters. At baseline, 1402 (37%) patients were included in cluster 1 and 2341(63%) in cluster 2. On day 3, 1557(42%) patients were included in cluster 1 and 2086 (57%) in cluster 2. The patients included in cluster 2 were older and more frequently hypertensive and had a higher prevalence of shock, organ dysfunction, inflammatory biomarkers, and worst respiratory indexes at both time points. The 90-day mortality was higher in cluster 2 at both clustering processes (43.8% [n = 1025] versus 27.3% [n = 383] at baseline, and 49% [n = 1023] versus 20.6% [n = 321] on day 3). Four hundred and fifty-eight (33%) patients clustered in the first group were clustered in the second group on day 3. In contrast, 638 (27%) patients clustered in the second group were clustered in the first group on day 3. Conclusions: During the first days, patients can be clustered into two groups and the process of clustering patients may change as they continue to evolve. This means that despite a vast majority of patients remaining in the same cluster, a minority reaching 33% of patients analyzed may be re-categorized into different clusters based on their progress. Such changes can significantly impact their prognosis.
  • Item
    Open Access
    Incidence, Clinicopathological Features and Oncologic Outcome of Appendiceal Neoplasms: A Single-Center Cohort Study
    (MDPI, 2023) Gómez-Báez, Ferney David; Cerdán Santacruz, Carlos; Moreno Muguiro, Naroa; Milla Collado, Lucía; Merichal Resina, Mireia; Tarragona Foradada, Jordi; Sierra Grañón, Enrique; Olsina Kissler, Jorge Juan
    Appendiceal tumors represent a large amalgam of different tumor lineages. The continuous evolution in their pathological classifications has led to some variable recommended attitudes over time. The aim of this study is to review the incidence, clinicopathological characteristics, therapeutic approach and oncological results in this type of tumor at our institution. This is a single centre retrospective cohort study. Every pathologic report catalogued as an appendiceal specimen was reviewed for a time period of 5 years (2013–2017) at our institution. Demographic, clinical, pathological and oncologic follow-up data were recorded. A descriptive study of the sample was completed. A total of 1434 appendiceal specimens was analyzed. Appendiceal neoplasms incidence was 3.2%. Epithelial tumors were the predominant histological subtype, making up 68% of the cases. Low-grade appendiceal mucinous neoplasia and neuroendocrine tumors were the most frequent neoplasms with malignant potential, with 13 and 6 cases, respectively. In more than 80% of neoplasia cases, the definitive treatment was appendectomy. Mortality cases were related to tumors with a very poor prognosis and an advanced stage. All patients had adequate oncological follow-up. Although it is still quite rare, the incidence of appendiceal tumors is increasing with an epidemiological change in favor of mucinous neoplasms currently predominating. Therefore, it is necessary to know and use an updated anatomo-pathological classification in order to provide correct treatment in the first or second surgical stage, as well as the correct follow-up of patients