Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy
dc.contributor.author | Martinez-Giner, Maria | |
dc.contributor.author | Noguera Jiménez, José Luis | |
dc.contributor.author | Balcells, Ingrid | |
dc.contributor.author | Fernandez-Rodriguez, Amanda | |
dc.contributor.author | Pena i Subirà, Ramona Natacha | |
dc.date.accessioned | 2014-06-12T14:51:49Z | |
dc.date.available | 2014-06-12T14:51:49Z | |
dc.date.issued | 2013 | |
dc.description.abstract | Reproductive traits play a key role in pig production in order to reduce costs and increase economic returns. Among others, gene expression analyses represent a useful approach to study genetic mechanisms underlying reproductive traits in pigs. The application of reverse-transcription quantitative PCR requires the selection of appropriate reference genes, whose expression levels should not be affected by the experimental conditions, especially when comparing gene expression across different physiological stages. | ca_ES |
dc.description.sponsorship | This study was funded by the Spanish Ministerio de Ciencia e Innovación (grant AGL2004-08368-C03/GAN). RN Pena received a contractual grant from INIA. M Martínez-Giner received a predoctoral fellowship from MICINN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0066023 | |
dc.identifier.idgrec | 019681 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issn | http://dx.doi.org/10.1371/journal.pone.0066023 | |
dc.identifier.uri | http://hdl.handle.net/10459.1/47316 | |
dc.language.iso | eng | ca_ES |
dc.publisher | Public Library of Science (PLoS) | ca_ES |
dc.relation | info:eu-repo/grantAgreement/MEC//AGL2004-08368-C03/ES/ | |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0066023 | ca_ES |
dc.relation.ispartof | PLoS ONE, 2014, vol.8, núm.6, e66023 | ca_ES |
dc.rights | cc-by, (c) Martínez-Giner et al., 2013 | ca_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject.other | Porcs -- Genètica | ca_ES |
dc.subject.other | Porcs -- Cria i desenvolupament -- Aspectes econòmics | ca_ES |
dc.subject.other | Porcs -- Reproducció | ca_ES |
dc.title | Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy | ca_ES |
dc.type | article | ca_ES |
dc.type.version | publishedVersion | ca_ES |