Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy

dc.contributor.authorMartinez-Giner, Maria
dc.contributor.authorNoguera Jiménez, José Luis
dc.contributor.authorBalcells, Ingrid
dc.contributor.authorFernandez-Rodriguez, Amanda
dc.contributor.authorPena i Subirà, Ramona Natacha
dc.date.accessioned2014-06-12T14:51:49Z
dc.date.available2014-06-12T14:51:49Z
dc.date.issued2013
dc.description.abstractReproductive traits play a key role in pig production in order to reduce costs and increase economic returns. Among others, gene expression analyses represent a useful approach to study genetic mechanisms underlying reproductive traits in pigs. The application of reverse-transcription quantitative PCR requires the selection of appropriate reference genes, whose expression levels should not be affected by the experimental conditions, especially when comparing gene expression across different physiological stages.ca_ES
dc.description.sponsorshipThis study was funded by the Spanish Ministerio de Ciencia e Innovación (grant AGL2004-08368-C03/GAN). RN Pena received a contractual grant from INIA. M Martínez-Giner received a predoctoral fellowship from MICINN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0066023
dc.identifier.idgrec019681
dc.identifier.issn1932-6203
dc.identifier.issnhttp://dx.doi.org/10.1371/journal.pone.0066023
dc.identifier.urihttp://hdl.handle.net/10459.1/47316
dc.language.isoengca_ES
dc.publisherPublic Library of Science (PLoS)ca_ES
dc.relationinfo:eu-repo/grantAgreement/MEC//AGL2004-08368-C03/ES/
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0066023ca_ES
dc.relation.ispartofPLoS ONE, 2014, vol.8, núm.6, e66023ca_ES
dc.rightscc-by, (c) Martínez-Giner et al., 2013ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subject.otherPorcs -- Genèticaca_ES
dc.subject.otherPorcs -- Cria i desenvolupament -- Aspectes econòmicsca_ES
dc.subject.otherPorcs -- Reproduccióca_ES
dc.titleSelection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancyca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
019681.pdf
Size:
460.01 KB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: