Iron and Zinc in the Embryo and Endosperm of Rice (Oryza sativa L.) Seeds in Contrasting 20-Deoxymugineic Acid/Nicotianamine Scenarios
| dc.contributor.author | Díaz-Benito, Pablo | |
| dc.contributor.author | Banakar, Raviraj | |
| dc.contributor.author | Rodríguez-Menéndez, Sara | |
| dc.contributor.author | Capell Capell, Teresa | |
| dc.contributor.author | Pereiro, Rosario | |
| dc.contributor.author | Christou, Paul | |
| dc.contributor.author | Abadía Bayona, Javier | |
| dc.contributor.author | Fernández, Beatriz | |
| dc.contributor.author | Álvarez-Fernández, Ana | |
| dc.date.accessioned | 2019-01-26T09:53:08Z | |
| dc.date.available | 2019-01-26T09:53:08Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Iron and Zn deficiencies are worldwide nutritional disorders that can be alleviated by increasing the metal concentration of rice (Oryza sativa L.) grains via bio-fortification approaches. The overproduction of the metal chelator nicotianamine (NA) is among the most effective ones, but it is still unclear whether this is due to the enrichment in NA itself and/or the concomitant enrichment in the NA derivative 20-deoxymugineic acid (DMA). The endosperm is the most commonly consumed portion of the rice grain and mediates the transfer of nutrients from vegetative tissues to the metal rich embryo. The impact of contrasting levels of DMA and NA on the metal distribution in the embryo and endosperm of rice seeds has been assessed using wild-type rice and six different transgenic lines overexpressing nicotianamine synthase (OsNAS1) and/or barley nicotianamine amino transferase (HvNAATb). These transgenic lines outlined three different DMA/NA scenarios: (i) in a first scenario, an enhanced NA level (via overexpression of OsNAS1) would not be fully depleted because of a limited capacity to use NA for DMA synthesis (lack of -or low- expression of HvNAATb), and results in consistent enrichments in NA, DMA, Fe and Zn in the endosperm and NA, DMA and Fe in the embryo; (ii) in a second scenario, an enhanced NA level (via overexpression of OsNAS1) would be depleted by an enhanced capacity to use NA for DMA synthesis (via expression of HvNAATb), and results in enrichments only for DMA and Fe, both in the endosperm and embryo, and (iii) in a third scenario, the lack of sufficient NA replenishment would limit DMA synthesis, in spite of the enhanced capacity to use NA for this purpose (via expression of HvNAATb), and results in decreases in NA, variable changes in DMA and moderate decreases in Fe in the embryo and endosperm. Also, quantitative LA-ICP-MS metal map images of the embryo structures show that the first and second scenarios altered local distributions of Fe, and to a lesser extent of Zn. The roles of DMA/NA levels in the transport of Fe and Zn within the embryo are thoroughly discussed. | ca_ES |
| dc.description.sponsorship | This work was supported by the grants of the Spanish Ministry of Science, Innovation and Universities (AGL2016-75226-R, BIO2014-54426-P and AGL2017-85377-R, all co-financed with FEDER), Aragón Government (Group A09_17R) and Generalitat de Catalunya (Grant 2017 SGR 828). PD-B was supported by a MINECO-FPI contract. RB was supported by a Ph.D. fellowship from the University of Lleida. SR-M was supported by a research contract from the Fundación Universidad de Oviedo (FUO-069-17). BF was supported by a MINECO research contract (RYC-2014-14985; “Ramón y Cajal Program”). | ca_ES |
| dc.identifier.doi | https://doi.org/10.3389/fpls.2018.01190 | |
| dc.identifier.idgrec | 027306 | |
| dc.identifier.issn | 1664-462X | |
| dc.identifier.uri | http://hdl.handle.net/10459.1/65647 | |
| dc.language.iso | eng | ca_ES |
| dc.publisher | Frontiers Media | ca_ES |
| dc.relation | info:eu-repo/grantAgreement/MINECO//AGL2016-75226-R/ES/ | ca_ES |
| dc.relation | info:eu-repo/grantAgreement/MINECO//BIO2014-54426-P/ES/UNA CELULA DE ARROZ MODERNIZADA PARA INVESTIGACIONES EN BIOLOGIA SINTETICA Y SUS APLICACIONES/ | ca_ES |
| dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85377-R/ES/IMPACTO DE LAS PRACTICAS DE CULTIVO EN PLANTAS DE ARROZ QUE ACUMULAN UNA COMBINACION DE MICROBICIDAS EN EL ENDOSPERMO DE SUS SEMILLAS/ | ca_ES |
| dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fpls.2018.01190 | ca_ES |
| dc.relation.ispartof | Frontiers in Plant Science, vol. 9, article 1190, p. 1-17 | ca_ES |
| dc.rights | cc-by (c) Díaz-Benito et al., 2018 | ca_ES |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Metals | ca_ES |
| dc.subject | Laser ablation | ca_ES |
| dc.subject | Ligands | ca_ES |
| dc.subject | Mass spectrometry | ca_ES |
| dc.title | Iron and Zinc in the Embryo and Endosperm of Rice (Oryza sativa L.) Seeds in Contrasting 20-Deoxymugineic Acid/Nicotianamine Scenarios | ca_ES |
| dc.type | info:eu-repo/semantics/article | ca_ES |
| dc.type.version | info:eu-repo/semantics/publishedVersion | ca_ES |