Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots

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dc.contributor.authorLópez-Gámez, Gloria
dc.contributor.authorElez Martínez, Pedro
dc.contributor.authorMartín Belloso, Olga
dc.contributor.authorSoliva-Fortuny, Robert
dc.date.accessioned2021-07-16T11:34:53Z
dc.date.available2022-07-10T22:06:52Z
dc.date.issued2020-07-10
dc.date.updated2021-07-16T11:34:53Z
dc.description.abstractPulsed electric fields (PEF) can be applied to induce accumulation of bioactive compounds in plant tissues to obtain commodities with health-promoting properties. However, causes of this accumulation are not fully un- derstood as it may result from either an improvement in extraction or an activation of stress-related biosynthetic pathways. The objective of this study was to investigate the effects of PEF on the physiological response and elucidating the causes underpinning changes in carrot phenolic contents. Respiration rate, oxidative and pec- tinolytic enzyme activities, synthesis, and content of phenolic compounds were evaluated in PEF-treated (580 J kg−1) carrots after treatment and through storage (12, 24 and 36 h) at 4 °C. The highest production of CO2 and volatile organic compounds was observed 12 h after PEF treatment whereas the largest increases in total phenolic content (80.2 %), p-OH-benzoic (94.7 %), chlorogenic acid (74.9 %) and ferulic acid (52.2 %) occurred 24 h after treatment. Enhanced in phenylalanine ammonia lyase activity indicated that the increase in phenolic compounds may be mainly due to the triggering of biosynthesis pathways instead of structural modifications of the food matrix. Electropermeabilization also induced considerable changes in pectinolytic enzyme activities (increases in pectinmethylesterase and decreases in polygalacturonase) whereas no clear trends were observed for oxidative enzyme activities (peroxidase and polyphenol oxidase) during storage. These results suggest that volatile compounds generation, changes in respiration rate and the biosynthesis of phenolic compounds are induced by PEF application, as a plant defence response to stress.
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy and Competitiveness (grant AGL2013-44851-R, 2013). Gloria López-Gámez thanks the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR) for the predoctoral grant.
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.postharvbio.2020.111284
dc.identifier.idgrec031333
dc.identifier.issn0925-5214
dc.identifier.urihttp://hdl.handle.net/10459.1/71668
dc.language.isoeng
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MINECO//AGL2013-44851-R/ES/ESTRATEGIAS INNOVADORAS PARA EL DESARROLLO DE ALIMENTOS VEGETALE
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.postharvbio.2020.111284
dc.relation.ispartofPostharvest Biology and Technology, 2020, vol. 168, 111284
dc.rightscc-by-nc-nd (c) Elsevier, 2020
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es
dc.subjectPulsed electric fields
dc.subjectPhenolic compounds
dc.subjectRespiration
dc.subjectPhenylalanine ammonia lyase
dc.titlePulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
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