Comparative functional analysis provides evidence for a crucial role for the homeobox gene Nkx2.1/Titf-1 in forebrain evolution

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dc.contributor.authorvan den Akker, Willem M.R.
dc.contributor.authorBrox, Aurora
dc.contributor.authorPuelles, Luis
dc.contributor.authorDurston, Antony J.
dc.contributor.authorMedina Hernández, Loreta Mª
dc.date.accessioned2017-01-19T11:49:48Z
dc.date.embargoEndDate2025-01-01
dc.date.issued2008
dc.description.abstractKnockout of the Nkx2.1 (Titf-1) homeobox gene in the mouse leads to severe malformation and size reduction of the basal telencephalon/preoptic area and basal hypothalamus, indicating an important role of this gene in forebrain patterning. Here we show that abrogation of the orthologous gene in the frog Xenopus laevis by way of morpholino knockdown also affects the relative size of major regions in both the telencephalon (subpallium versus pallium) and diencephalon (hypothalamus versus thalamus). Remarkably, while a similar effect on the telencephalon was noted previously in Nkx2.1-knockout mice, the effect on the diencephalon seems to be specific for Xenopus. This difference may be explained by the partially dissimilar expression of the orthologous genes in the forebrain of Xenopus and mouse. In both species Nkx2.1 is expressed in the basal telencephalon/preoptic area and basal hypothalamus, but in Xenopus this gene is additionally expressed in the alar hypothalamus. Phylogenetic comparison of Nkx2.1 expression in the forebrain suggests that the expression in the basal telencephalon-preoptic region and alar hypothalamus appeared in the transition from jawless to jawed vertebrates, but the alar hypothalamic expression was later dramatically reduced during evolution to birds and mammals. Our study suggests that changes in the regulation of Nkx2.1 expression have played an important role on the evolution of forebrain development, and emphasizes the potential of the combined analysis of expression and function of master control genes in different vertebrates for unraveling the origin of brain complexity and diversity.ca_ES
dc.description.sponsorshipGrant sponsor: Spanish Ministry of Education and Science; Grant numbers: DGICYT-FEDER BFI2003-06453-C02-02 and BFU2006-14804-C02-02/BFI (to L.M.); Grant sponsor: Se´neca Foundation; Grant number: PB/50/FS/02; Grant sponsors: Se´neca Foundation and EMBO (fellowships to A.B.).ca_ES
dc.identifier.doihttps://doi.org/10.1002/cne.21542
dc.identifier.idgrec011379
dc.identifier.issn0021-9967
dc.identifier.urihttp://hdl.handle.net/10459.1/59042
dc.language.isoengca_ES
dc.publisherWileyca_ES
dc.relationMICYT/PN2000-2003/BFI2003-06453-C02-02
dc.relationMIECI/PN2004-2007/BFU2006-14804-C02-02/BFI
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1002/cne.21542ca_ES
dc.relation.ispartofThe Journal of Comparative Neurology, 2008, vol. 506, núm. 2, p. 211–223ca_ES
dc.rights(c) Wiley-Liss, Inc. 2007ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.subjectXenopusca_ES
dc.subjectTelencephalonca_ES
dc.subjectCortex evolutionca_ES
dc.subjectThalamusca_ES
dc.titleComparative functional analysis provides evidence for a crucial role for the homeobox gene Nkx2.1/Titf-1 in forebrain evolutionca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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