Calmodulin Modulates Mitogen-Activated Protein Kinase Activation in Response to Membrane Depolarization in PC12 Cells

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dc.contributor.authorEgea Navarro, Joaquim
dc.contributor.authorEspinet Mestre, Carme
dc.contributor.authorComella i Carnicé, Joan Xavier
dc.date.accessioned2016-05-05T08:23:32Z
dc.date.embargoEndDate2025-01-01
dc.date.issued1998
dc.description.abstractIn the absence of neurotrophic factors, chronic depolarization of plasma membrane has been shown to maintain several populations of primary neurons in culture. We report that in the PC12 cell line, depolarization causes Ca2+ influx through voltage-gated Ca2+ channels, which is able to stimulate extracellular-regulated kinase (ERK) activity. We studied which mediators were responsible for ERK activation resulting from increased levels of Ca2+ in the cytoplasm and found that calmodulin was involved in this process. The addition of W13, a calmodulin inhibitor, to the culture medium, prevented ERK activation when PC12 cells were depolarized. In addition, we show that high K+ treatment did not induce Trk A phosphorylation, thus excluding the possibility of Ca2+ operating through this receptor to activate the ERK signal transduction pathway. Moreover, although high K+ treatment is able to phosphorylate the epidermal growth factor receptor (EGFR) and thus to activate the ERK signal transduction pathway, we demonstrate that W13 did not alter the state of EGFR phosphorylation in conditions that almost completely blocked ERK activation. These data suggest that calmodulin mediates ERK activation induced by increases in intracellular Ca2+ concentration in PC12 cells by a mechanism that seems to be independent of Trk A and EGFR activation.ca_ES
dc.identifier.doihttps://doi.org/10.1046/j.1471-4159.1998.70062554.x
dc.identifier.idgrec000368
dc.identifier.issn0022-3042
dc.identifier.urihttp://hdl.handle.net/10459.1/56952
dc.language.isoengca_ES
dc.publisherWileyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1046/j.1471-4159.1998.70062554.xca_ES
dc.relation.ispartofJournal of Neurochemistry, 1998, vol. 70, núm. 6, p. 2554-2564ca_ES
dc.rights(c) International Society for Neurochemistry, 1998ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.subjectNeurotrophismca_ES
dc.subjectTyrosine kinase receptorca_ES
dc.subjectCalciumca_ES
dc.subjectNeuronca_ES
dc.titleCalmodulin Modulates Mitogen-Activated Protein Kinase Activation in Response to Membrane Depolarization in PC12 Cellsca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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