ZmPBF and ZmGAMYB transcription factors independently transactivate the promoter of the maize (Zea mays L.) β-carotene hydroxylase 2 gene

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dc.contributor.authorJin, Xin
dc.contributor.authorBai, Chao
dc.contributor.authorBassie Rene, Ludovic
dc.contributor.authorNogareda, Carmina
dc.contributor.authorRomagosa Clariana, Ignacio
dc.contributor.authorTwyman, Richard M.
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.date.accessioned2019-01-21T11:50:32Z
dc.date.available2019-11-29T23:18:17Z
dc.date.issued2018-12-01
dc.date.updated2019-01-21T11:50:32Z
dc.description.abstractThe maize (Zea mays) enzyme b-carotene hydroxylase 2 (ZmBCH2) controls key steps in the conversion of b-carotene to zeaxanthin in the endosperm. The ZmBCH2 gene has an endosperm-preferred and developmentally regulated expression profile, but the detailed regulatory mechanism is unknown. To gain insight into the regulation of ZmBCH2, we isolated 2036 bp of the 50-flanking region containing the 263 bp 50-untranslated region (50-UTR) including the first intron. We linked this to the b-glucuronidase reporter gene gusA. We found that high-level expression of gusA in rice seeds requires the 50-UTR for enhanced activation. Truncated variants of the ZmBCH2 promoter retained their seed-preferred expression profile as long as a prolamin box and AACA motif were present. We identified candidate genes encoding the corresponding transcription factors (ZmPBF and ZmGAMYB) and confirmed that their spatiotemporal expression profiles are similar to ZmBCH2. Both ZmPBF and ZmGAMYB can transactivate ZmBCH2 expression in maize endosperm. To eliminate potential confounding effects in maize, we characterized the regulation of the minimal promoter region of ZmBCH2 in transgenic rice. This revealed that ZmPBF and ZmGAMYB independently transactivate the ZmBCH2 promoter. The mechanism that underpins our data provides an exciting new strategy for the control of target gene expression in engineered plants.
dc.description.sponsorshipThis work was supported by MINECO (Ministry of Economy and Competitiveness), Spain BIO2014-54441-P; BIO2014-54426-P) and the National Natural Science Foundation of China (31470394).
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/nph.15614
dc.identifier.idgrec028168
dc.identifier.issn0028-646X
dc.identifier.urihttp://hdl.handle.net/10459.1/65582
dc.language.isoeng
dc.publisherWiley
dc.relationinfo:eu-repo/grantAgreement/MINECO//BIO2014-54441-P/ES/REGULACION DE LA BIOSINTESIS Y ACUMULACION DE CAROTENOIDES EN EL ENDOSPERMO DE MAIZ/
dc.relationinfo:eu-repo/grantAgreement/MINECO//BIO2014-54426-P/ES/UNA CELULA DE ARROZ MODERNIZADA PARA INVESTIGACIONES EN BIOLOGIA SINTETICA Y SUS APLICACIONES/
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1111/nph.15614
dc.relation.ispartofNew Phytologist, 2018, vol. 11, p. 1-14
dc.rights(c) Jin et al., 2018
dc.rights(c) New Phytologist Trust, 2018
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subject5'-untranslated region
dc.subjectAACA motif
dc.subjectCarotenoids
dc.subjectCis-regulatory elements
dc.titleZmPBF and ZmGAMYB transcription factors independently transactivate the promoter of the maize (Zea mays L.) β-carotene hydroxylase 2 gene
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
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