Plasma-Functionalized Isotactic Polypropylene Assembledwith Conducting Polymers for Bacterial Quantification byNADH Sensing

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dc.contributor.authorMolina, Brenda G.
dc.contributor.authordel Valle, Luis J.
dc.contributor.authorCasanovas Salas, Jordi
dc.contributor.authorLanzalaco, Sonia
dc.contributor.authorPérez-Madrigal, Maria M.
dc.contributor.authorTuron, Pau
dc.contributor.authorArmelin, Elaine
dc.contributor.authorAlemán, Carlos
dc.date.accessioned2022-11-13T12:29:53Z
dc.date.available2022-11-13T12:29:53Z
dc.date.issued2021
dc.description.abstractRapid detection of bacterial presence on implantable medical devices isessential to prevent biofilm formation, which consists of densely packedbacteria colonies able to withstand antibiotic-mediated killing. In this work, asmart approach is presented to integrate electrochemical sensors fordetecting bacterial infections in biomedical implants made of isotacticpolypropylene (i-PP) using chemical assembly. The electrochemical detectionis based on the capacity of conducting polymers (CPs) to detect extracellularnicotinamide adenine dinucleotide (NADH) released from cellular respirationof bacteria, which allows distinguishing prokaryotic from eukaryotic cells.Oxygen plasma-functionalized free-standing i-PP, coated with a layer(≈1.1 μm in thickness) of CP nanoparticles obtained by oxidativepolymerization, is used as working electrode for the anodic polymerization ofa second CP layer (≈8.2 μm in thickness), which provides very highelectrochemical activity and stability. The resulting layered material, i-PPf/CP2,detects the electro-oxidation of NADH in physiological media with asensitivity 417 μA cm−2and a detection limit up to 0.14×10−3m, which isbelow the concentration of extracellular NADH found for bacterial cultures ofbiofilm-positive and biofilm-negative strains.ca_ES
dc.description.sponsorshipAuthors acknowledge MINECO/FEDER (RTI2018-098951-B-I00), the Agència de Gestió d’Ajuts Universitaris i de Recerca (2017SGR359), and B. Braun Surgical, S.A. for financial support. Support for the research of C.A. from ICREA Academia program for excellence in research is gratefully acknowledged.
dc.identifier.doihttps://doi.org/10.1002/adhm.202100425
dc.identifier.idgrec030614
dc.identifier.issn2192-2640
dc.identifier.urihttp://hdl.handle.net/10459.1/84209
dc.language.isoengca_ES
dc.publisherWiley-VCH Verlagca_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098951-B-I00/ES/DISPOSITIVOS AUTONOMOS PARA LA DETECCION Y LA LIBERACION: ENSAMBLAJE DE SISTEMAS BIOMEDICOS PARA EL DIAGNOSTICO Y LA TERAPIA/ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1002/adhm.202100425ca_ES
dc.relation.ispartofAdvanced Healthcare Materials, 2021, vol. 10, 2100425ca_ES
dc.rightscc-by-nc-nd (c) Wiley-VCH GmbH, 2021ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBacteria respirationca_ES
dc.subjectBacteria sensorsca_ES
dc.subjectBiomedical implantsca_ES
dc.subjectFlexible sen-sorsca_ES
dc.subjectPoly(3,4-ethylenedioxythiophene)ca_ES
dc.titlePlasma-Functionalized Isotactic Polypropylene Assembledwith Conducting Polymers for Bacterial Quantification byNADH Sensingca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_ES
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