Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway

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dc.contributor.authorIzquierdo Álvarez, Alicia
dc.contributor.authorCasas Herranz, Celia
dc.contributor.authorMühlenhoff, Ulrich
dc.contributor.authorLillig, Christopher Horst
dc.contributor.authorHerrero Perpiñán, Enrique
dc.date.accessioned2014-05-19T15:48:34Z
dc.date.available2014-05-19T15:48:34Z
dc.date.issued2008
dc.description.abstractSaccharomyces cerevisiae Grx6 and Grx7 are two monothiol glutaredoxins whose active-site sequences (CSYS and CPYS, respectively) are reminiscent of the CPYC active-site sequence of classical dithiol glutaredoxins. Both proteins contain an N-terminal transmembrane domain which is responsible for their association to membranes of the early secretory pathway vesicles, facing the luminal side. Thus, Grx6 localizes at the endoplasmic reticulum and Golgi compartments, while Grx7 is mostly at the Golgi. Expression of GRX6 is modestly upregulated by several stresses (calcium, sodium, and peroxides) in a manner dependent on the Crz1-calcineurin pathway. Some of these stresses also upregulate GRX7 expression under the control of the Msn2/4 transcription factor. The N glycosylation inhibitor tunicamycin induces the expression of both genes along with protein accumulation. Mutants lacking both glutaredoxins display reduced sensitivity to tunicamycin, although the drug is still able to manifest its inhibitory effect on a reporter glycoprotein. Grx6 and Grx7 have measurable oxidoreductase activity in vivo, which is increased in the presence of tunicamycin. Both glutaredoxins could be responsible for the regulation of the sulfhydryl oxidative state at the oxidant conditions of the early secretory pathway vesicles. However, the differences in location and expression responses against stresses suggest that their functions are not totally overlapping.ca_ES
dc.identifier.doihttps://doi.org/10.1128/EC.00133-08
dc.identifier.idgrec013102
dc.identifier.issn1535-9778 (versió paper)
dc.identifier.issn1535-9786
dc.identifier.urihttp://hdl.handle.net/10459.1/47223
dc.language.isoengca_ES
dc.publisherAmerican Society for Microbiologyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1128/EC.00133-08ca_ES
dc.relation.ispartofEukaryotic cell, 2008, vol. 7, núm. 8, 9. 1415-1426ca_ES
dc.rights(c) American Society for Microbiologyca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.subjectLlevat de cervesaca_ES
dc.subjectGrx6ca_ES
dc.subjectGrx7ca_ES
dc.subject.otherSaccharomyces cerevisiaeca_ES
dc.subject.otherBiologia molecularca_ES
dc.titleSaccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathwayca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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