Recruitment of Staufen2 Enhances Dendritic Localization of an Intron-Containing CaMKIIα mRNA

dc.contributor.authorOrtiz, Raul
dc.contributor.authorGeorgieva, Maya V.
dc.contributor.authorGutiérrez, Sara
dc.contributor.authorPedraza González, Neus
dc.contributor.authorFernández-Moya, Sandra M.
dc.contributor.authorGallego, Carme
dc.date.accessioned2017-10-16T10:03:31Z
dc.date.available2017-10-16T10:03:31Z
dc.date.issued2017
dc.description.abstractRegulation of mRNA localization is a conserved cellular process observed in many types of cells and organisms. Asymmetrical mRNA distribution plays a particularly important role in the nervous system, where local translation of localized mRNA represents a key mechanism in synaptic plasticity. CaMKIIα is a very abundant mRNA detected in neurites, consistent with its crucial role at glutamatergic synapses. Here, we report the presence of CaMKIIα mRNA isoforms that contain intron i16 in dendrites, RNA granules, and synaptoneurosomes from primary neurons and brain. This subpopulation of unspliced mRNA preferentially localizes to distal dendrites in a synaptic-activity-dependent manner. Staufen2, a well-established marker of RNA transport in dendrites, interacts with intron i16 sequences and enhances its distal dendritic localization, pointing to the existence of intron-mediated mechanisms in the molecular pathways that modulate dendritic transport and localization of synaptic mRNAs.ca_ES
dc.description.sponsorshipThis work was funded by grants from the Ministry of Economy and Competitiveness of Spain (BFU2014-52591-R CG) and the European Union (FEDER) to C.G.ca_ES
dc.identifier.doihttps://doi.org/10.1016/j.celrep.2017.06.026
dc.identifier.issn2211-1247
dc.identifier.urihttp://hdl.handle.net/10459.1/60300
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO//BFU2014-52591-R/ES/PLASTICIDAD SINAPTICA Y ENFERMEDADES NEUROLOGICAS: LA ISOFORMA EEF1A2 DEL FACTOR DE ELONGACION DE CEREBRO/
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.celrep.2017.06.026ca_ES
dc.relation.ispartofCell Reports, 2017, vol. 20, núm. 1, p. 13-20ca_ES
dc.rightscc-by-nc-nd (c) Ortiz et al., 2017ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectStau2ca_ES
dc.subjectIntron retentionca_ES
dc.subjectCaMKIIαca_ES
dc.subjectmRNA transportca_ES
dc.titleRecruitment of Staufen2 Enhances Dendritic Localization of an Intron-Containing CaMKIIα mRNAca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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