Serum Deprivation and Protein Synthesis Inhibition Induce Two Different Apoptotic Processes in N18 Neuroblastoma Cells

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dc.contributor.authorFibla Palazón, Joan
dc.contributor.authorYuste Mateos, Víctor J. (Víctor José)
dc.contributor.authorPiulats, J.M
dc.contributor.authorLlecha Cano, Núria
dc.contributor.authorComella i Carnicé, Joan Xavier
dc.contributor.authorBoix Torras, Jacint
dc.date.accessioned2016-05-05T08:44:32Z
dc.date.embargoEndDate2025-01-01
dc.date.issued1998
dc.description.abstractN18 are murine neuroblastoma cells that underwent cell death upon serum deprivation or inhibition of protein synthesis by means of cycloheximide (CHX). In both cases, an ultrastructural morphology and an internucleosomal pattern of DNA fragmentation typical of apoptosis were found. However, electron microscopy revealed abundant lipid vesicles in the cytoplasm of CHX-treated cells that were not found in their serum-deprived counterparts. In addition, when both types of apoptotic cells were compared by means of flow cytometry and chromatin staining with propidium iodide, the former showed consistently less fluorescence than the latter. Therefore, in N18 cells, both apoptotic processes seemed to differ at a structural level. At a functional level, we found that apoptosis was blocked by the protease inhibitor TLCK in CHX-treated but not in serum-deprived cells. On the other hand, we generated N18 clones that overexpressed Bcl-2 protein. After a period of 48 h we found that identical levels of Bcl-2 protein were able to block apoptosis in serum-deprived but not in CHX-treated cells. In conclusion, two different biochemical pathways leading to apoptosis seem to coexist in N18 neuroblastoma cells.ca_ES
dc.identifier.doihttps://doi.org/10.1006/excr.1997.3852
dc.identifier.idgrec000366
dc.identifier.issn0014-4827
dc.identifier.urihttp://hdl.handle.net/10459.1/56953
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1006/excr.1997.3852ca_ES
dc.relation.ispartofExperimental Cell Research, 1998, vol. 238, núm. 2, p. 422-429ca_ES
dc.rights(c) Academic Press, 1998ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.subjectApoptosisca_ES
dc.subjectNeuroblastomaca_ES
dc.subjectProtease inhibitorsca_ES
dc.subjectBcl-2ca_ES
dc.titleSerum Deprivation and Protein Synthesis Inhibition Induce Two Different Apoptotic Processes in N18 Neuroblastoma Cellsca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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