The coordinated upregulated expression of genes involved in MEP, chlorophyll, carotenoid and tocopherol pathways, mirrored the corresponding metabolite contents in rice leaves during de-etiolation

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dc.contributor.authorJin, Xin
dc.contributor.authorBaysal, Can
dc.contributor.authorDrapal, Margit
dc.contributor.authorSheng, Yanmin
dc.contributor.authorHuang, Xin
dc.contributor.authorHe, Wenshu
dc.contributor.authorShi, Lianxuan
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorFraser, Paul
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.date.accessioned2021-10-11T09:16:20Z
dc.date.available2021-10-11T09:16:20Z
dc.date.issued2021-07-16
dc.date.updated2021-10-11T09:16:20Z
dc.description.abstractLight is an essential regulator of many developmental processes in higher plants. We investigated the effect of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1/2 genes (OsHDR1/2) and isopentenyl diphosphate isomerase 1/2 genes (OsIPPI1/2) on the biosynthesis of chlorophylls, carotenoids, and phytosterols in 14-day-old etiolated rice (Oyza sativa L.) leaves during de-etiolation. However, little is known about the effect of isoprenoid biosynthesis genes on the corresponding metabolites during the de-etiolation of etiolated rice leaves. The results showed that the levels of α-tocopherol were significantly increased in de-etiolated rice leaves. Similar to 1-deoxy-D-xylulose-5-phosphate synthase 3 gene (OsDXS3), both OsDXS1 and OsDXS2 genes encode functional 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activities. Their expression patterns and the synthesis of chlorophyll, carotenoid, and tocopherol metabolites suggested that OsDXS1 is responsible for the biosynthesis of plastidial isoprenoids in de-etiolated rice leaves. The expression analysis of isoprenoid biosynthesis genes revealed that the coordinated expression of the MEP (2-C-methyl-D-erythritol 4-phosphate) pathway, chlorophyll, carotenoid, and tocopherol pathway genes mirrored the changes in the levels of the corresponding metabolites during de-etiolation. The underpinning mechanistic basis of coordinated light-upregulated gene expression was elucidated during the de-etiolation process, specifically the role of light-responsive cis-regulatory motifs in the promoter region of these genes. In silico promoter analysis showed that the light-responsive cis-regulatory elements presented in all the promoter regions of each light-upregulated gene, providing an important link between observed phenotype during de-etiolation and the molecular machinery controlling expression of these genes
dc.description.sponsorshipThis research was funded by Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University (Project No. GSCS-2020-07); The National Natural Science Foundation of China (31870278); the Spanish Ministry of Economy and Competitiveness (MINECO), Spain (RTI2018-097613-B-I00; PGC2018-097655-B-I00); in part by the European Union Framework Pro- gram DISCO (613513) “from DISCOvery to products: a next-generation pipeline for the sustainable generation of high-value plant products”, the European Cooperation in Science and Technology project EUROCAROTEN (OC-2015-1-19780), Generalitat de Catalunya Grant 2017 SGR 828 to the Agricultural Biotechnology and Bioeconomy Unit (ABBU), and the International Science and Technol- ogy Cooperation Project 20190201013JC (from Jilin Provincial Science and Technology Department, China); GAU-KYQD-2019-06, Gansu Agricultural University.
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3390/plants10071456
dc.identifier.idgrec031656
dc.identifier.issn2223-7747
dc.identifier.urihttp://hdl.handle.net/10459.1/72034
dc.language.isoeng
dc.publisherMDPI
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097613-B-I00/ES/ELUCIDACION DEL MECANISMO RESPONSABLE DE LA ACUMULACION Y ESTABILIDAD DEL B-CAROTENO EN EL ENDOSPERMO DEL MAIZ/
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097655-B-I00/ES/INVESTIGAR EL MECANISMO DE REACTIVACION DE PROMOTORES QUE SE ENCUENTRAN LATENTES EN EL ENDOSPERMO PARA SU USO EN INGENIERIA METABOLICA RESULTANDO EN PLANTAS NO TRANSGENICAS/
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3390/plants10071456
dc.relation.ispartofPlants, 2021, vol. 10, p. 1456-1471
dc.relation.projectIDInfo:eu-repo/grantAgreement/EC/FP7/613513
dc.rightscc-by (c) Jin et al., 2021
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.source.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCarotenoids
dc.subjectCoordinated gene expression
dc.subjectLight-upregulated genes
dc.subjectIsoprenoids
dc.subjectRice (Oryza sativa L.)
dc.titleThe coordinated upregulated expression of genes involved in MEP, chlorophyll, carotenoid and tocopherol pathways, mirrored the corresponding metabolite contents in rice leaves during de-etiolation
dc.typeinfo:eu-repo/semantics/article
dc.type.versionpublishedVersion
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