Systematic Investigation of the Physicochemical Factors That Contribute to the Toxicity of ZnO Nanoparticles

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dc.contributor.authorMu, Qingshan
dc.contributor.authorDavid, Calin
dc.contributor.authorGalceran i Nogués, Josep
dc.contributor.authorRey Castro, Carlos
dc.contributor.authorKrzemiński, Łukasz
dc.contributor.authorWallace, Rachel
dc.contributor.authorBamiduro, Faith
dc.contributor.authorMilne, Steven J.
dc.contributor.authorHondow, Nicole S.
dc.contributor.authorBrydson, Rik
dc.contributor.authorVizcay-Barrena, Gema
dc.contributor.authorRoutledge, Michael N.
dc.contributor.authorJeuken, Lars J. C.
dc.contributor.authorBrown, Andy P.
dc.date.accessioned2015-11-25T09:47:14Z
dc.date.embargoEndDate2025-01-01
dc.date.issued2014
dc.description.abstractZnO nanoparticles (NPs) are prone to dissolution, and uncertainty remains whether biological/cellular responses to ZnO NPs are solely due to the release of Zn2+ or whether the NPs themselves have additional toxic effects. We address this by establishing ZnO NP solubility in dispersion media (Dulbecco’s modified Eagle’s medium, DMEM) held under conditions identical to those employed for cell culture (37 °C, 5% CO2, and pH 7.68) and by systematic comparison of cell–NP interaction for three different ZnO NP preparations. For NPs at concentrations up to 5.5 μg ZnO/mL, dissolution is complete (with the majority of the soluble zinc complexed to dissolved ligands in the medium), taking ca. 1 h for uncoated and ca. 6 h for polymer coated ones. Above 5.5 μg/mL, the results are consistent with the formation of zinc carbonate, keeping the solubilized zinc fixed to 67 μM of which only 0.45 μM is as free Zn2+, i.e., not complexed to dissolved ligands. At these relatively high concentrations, NPs with an aliphatic polyether-coating show slower dissolution (i.e., slower free Zn2+ release) and reprecipitation kinetics compared to those of uncoated NPs, requiring more than 48 h to reach thermodynamic equilibrium. Cytotoxicity (MTT) and DNA damage (Comet) assay dose–response curves for three epithelial cell lines suggest that dissolution and reprecipitation dominate for uncoated ZnO NPs. Transmission electron microscopy combined with the monitoring of intracellular Zn2+ concentrations and ZnO–NP interactions with model lipid membranes indicate that an aliphatic polyether coat on ZnO NPs increases cellular uptake, enhancing toxicity by enabling intracellular dissolution and release of Zn2+. Similarly, we demonstrate that needle-like NP morphologies enhance toxicity by apparently frustrating cellular uptake. To limit toxicity, ZnO NPs with nonacicular morphologies and coatings that only weakly interact with cellular membranes are recommended.ca_ES
dc.description.sponsorshipThe work leading to these results has received funding from the European Union Seventh Framework Programme (FP7-NMP-2008-1.3-2) under grant agreement no. 229244. A.P.B. holds an EPSRC ARF (EP/E059678/1). C.A.D., J.G., and C.R.C. also received support from the European Union Seventh Framework Programme (FP7-NMP.2012.1.3-3) under grant agreement no. 310584 (NANoREG)
dc.identifier.doihttps://doi.org//10.1021/tx4004243
dc.identifier.idgrec021160
dc.identifier.issn0893-228X
dc.identifier.urihttp://hdl.handle.net/10459.1/49048
dc.language.isoengca_ES
dc.publisherAmerican Chemical Societyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org//10.1021/tx4004243ca_ES
dc.relation.ispartofChemical Research in Toxicology, 2014, vol. 27, núm. 4, p. 558-567ca_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/229244ca_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/310584
dc.rights(c) American Chemical Society, 2014ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.subject.otherNanopartículesca_ES
dc.subject.otherQuímicaca_ES
dc.titleSystematic Investigation of the Physicochemical Factors That Contribute to the Toxicity of ZnO Nanoparticlesca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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