Accumulation of target gene mutations confers multiple resistance to ALS, ACCase and EPSPS inhibitors in Lolium species in Chile

dc.contributor.authorVázquez-García, José G.
dc.contributor.authorAlcántara-de la Cruz, Ricardo
dc.contributor.authorPalma-Bautista, Candelario
dc.contributor.authorRojano-Delgado, Antonia M.
dc.contributor.authorCruz-Hipólito, Hugo E.
dc.contributor.authorTorra Farré, Joel
dc.contributor.authorBarro, Francisco
dc.contributor.authorPrado, Rafael de
dc.description.abstractDifferent Lolium species, common weeds in cereal fields and fruit orchards in Chile, were reported showing isolated resistance to the acetyl CoA carboxylase (ACCase), acetolactate synthase (ALS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibiting herbicides in the late 1990s. The first case of multiple resistance to these herbicides was Lolium multiflorum found in spring barley in 2007. We hypothesized that other Lolium species may have evolved multiple resistance. In this study, we characterised the multiple resistance to glyphosate, diclofop-methyl and iodosulfuron-methyl-sodium in Lolium rigidum, Lolium perenne and Lolium multiflorum resistant (R) populations from Chile collected in cereal fields. Lolium spp. populations were confirmed by AFLP analysis to be L. rigidum, L. perenne and L. multiflorum. Dose-response assays confirmed multiple resistance to glyphosate, diclofop-methyl and iodosulfuron methyl-sodium in the three species. Enzyme activity assays (ACCase, ALS and EPSPS) suggested that the multiple resistance of the three Lolium spp. was caused by target site mechanisms, except the resistance to iodosulfuron in the R L. perenne population. The target site genes sequencing revealed that the R L. multiflorum population presented the Pro-106-Ser/Ala (EPSPS), Ile-2041-Asn+Asp-2078-Gly (ACCase), and Trp-574-Leu (ALS) mutations; and the R L. rigidum population had the Pro-106-Ser (EPSPS), Ile-1781-Leu+Asp-2078-Gly (ACCase) and Pro-197-Ser/Gln+Trp-574-Leu (ALS) mutations. Alternatively, the R L. perenne population showed only the Asp-2078-Gly (ACCase) mutation, while glyphosate resistance could be due to EPSPS gene amplification (no mutations but high basal enzyme activity), whereas iodosulfuron resistance presumably could involve non-target site resistance (NTSR) mechanisms. These results support that the accumulation of target site mutations confers multiple resistance to the ACCase, ALS and EPSPS inhibitors in L. multiflorum and L. rigidum from Chile, while in L. perenne, both target and NTSR could be present. Multiple resistance to three herbicide groups in three different species of the genus Lolium in South America represents a significant management challenge.
dc.description.sponsorshipThis work was funded by the Asociación de Agroquímicos y Medioambiente. The Spanish Ministry of Science and Innovation (Project PID2019-110847RB-I00) and the European Regional Development Fund (FEDER) supported this research. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). JT acknowledges support from the Spanish Ministry of Science, Innovation and Universities (grant Ramon y Cajal RYC2018-023866-I) and RA thanks support to the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2018/15910-6).
dc.publisherFrontiers Media
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110847RB-I00/ES/REDISEÑO DE LAS PROTEINAS INMUNOGENICAS DE TRIGO RELACIONADAS CON LA CELIAQUIA MEDIANTE CRISPR%2FCAS/
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofFrontiers In Plant Science, 2018, vol. 11, p. 553948
dc.rightscc-by (c) Vazquez-Garcia, José G. et al., 2020
dc.subjectIodosulfuron methyl-sodium
dc.subjectItalian ryegrass
dc.subjectPerennial ryegrass
dc.subjectRigid ryegrass
dc.titleAccumulation of target gene mutations confers multiple resistance to ALS, ACCase and EPSPS inhibitors in Lolium species in Chile
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