N-methyl-D-aspartate receptors are expressed in rat parathyroid gland and regulate PTH secretion

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dc.contributor.authorParisi Capdevila, Eva
dc.contributor.authorAlmadén, Yolanda
dc.contributor.authorIbarz Escuer, Mercedes
dc.contributor.authorPanizo García, Sara
dc.contributor.authorCardús i Figueras, Anna
dc.contributor.authorRodríguez, Mariano
dc.contributor.authorFernández i Giráldez, Elvira
dc.contributor.authorValdivielso Revilla, José Manuel
dc.date.accessioned2016-06-08T07:47:16Z
dc.date.embargoEndDate2025-01-01
dc.date.issued2009
dc.description.abstractN-methyl-d-aspartate receptors (NMDAR) are tetrameric amino acid receptors which act as membrane calcium channels. The presence of the receptor has been detected in the principal organs responsible for calcium homeostasis (kidney and bone), pointing to a possible role in mineral metabolism. In the present work, the presence of the receptor was determined in normal parathyroid glands (PTG) by real-time PCR, immunoprecipitation, and immunohistrochemistry. Healthy animals showed a decrease in blood parathyroid hormone (PTH) levels 15 min after the treatment with NMDA. This effect was also observed in animals with high levels of PTH-induced EDTA injection, but not in uremic animals with secondary hyperparathyroidism (2HPT). Normal rat PTG incubated in media with low calcium concentration (0.8 mM CaCl2) showed a decrease in PTH release when NMDA was added to the media. This effect of NMDA was abolished when glands were coincubated with MK801 (a pharmacological blocker of the NMDA channel) or PD98059 (an inhibitor of the ERK-MAPK pathway). Glands obtained from animals with 2HPT showed no effect of NMDA in the in vitro release of PTH, together with a decrease in the expression of NMDAR1. In conclusion, NMDA receptor is present in PTG and is involved in the regulation of the PTH release. The mechanism by which NMDAR exerts its function is through the activation of the MAPK cascade. In uremic 2HPT animals the receptor expression is downregulated and the treatment with NMDA does not affect PTH secretion.ca_ES
dc.description.sponsorshipThis work was supported by Fundació Dr. Pifarré grants, FIS PI07/0427 and REDINREN (16/06). J. M. Valdivielso holds a contract from the Miguel Servet program.ca_ES
dc.identifier.doihttps://doi.org/10.1152/ajprenal.90557.2008
dc.identifier.idgrec017039
dc.identifier.issn1931-857X
dc.identifier.urihttp://hdl.handle.net/10459.1/57172
dc.language.isoengca_ES
dc.publisherAmerican Physiological Societyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1152/ajprenal.90557.2008ca_ES
dc.relation.ispartofAJP - Renal Physiology, 2009, vol. 296, núm. 6, p. 1291-1296ca_ES
dc.rights(c) American Physiological Society, 2009ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.subjectSecondary hyperparathyroidismca_ES
dc.subjectERK-MAPKca_ES
dc.titleN-methyl-D-aspartate receptors are expressed in rat parathyroid gland and regulate PTH secretionca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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