Revealing genomic insights of the unexplored porcine pathogen actinobacillus pleuropneumoniae using whole genome sequencing

dc.contributor.authorGuitart-Matas, Judith
dc.contributor.authorGonzalez-Escalona, Narjol
dc.contributor.authorMaguire, Meghan
dc.contributor.authorVilaró, Anna
dc.contributor.authorMartínez-Urtaza, Jaime
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorMigura-García, Lourdes
dc.date.accessioned2022-11-30T10:09:18Z
dc.date.available2022-11-30T10:09:18Z
dc.date.issued2022
dc.description.abstractActinobacillus pleuropneumoniae (APP) is the causative agent of pleuropneumonia in pigs, one of the most relevant bacterial respiratory diseases in the swine industry. To date, 19 serotypes have been described based on capsular polysaccharide typing with significant virulence dissimilarities. In this study, 16 APP isolates from Spanish origin were selected to perform antimicrobial susceptibility tests and comparative genomic analysis using whole genome sequencing (WGS). To obtain a more comprehensive worldwide molecular epidemiologic analyses, all APP whole genome assemblies available at the National Center for Biotechnology Information (NCBI) at the time of the study were also included. An in-house in silico PCR approach enabled the correct serotyping of unserotyped or incorrectly serotyped isolates and allowed for the discrimination between serotypes 9 and 11. A pangenome analysis identified the presence or absence of gene clusters to be serotype specific, as well as virulence profile analyses targeting the apx operons. Antimicrobial resistance genes were correlated to the presence of specific plasmids. Altogether, this study provides new insights into the genetic variability within APP serotypes, correlates phenotypic tests with bioinformatic analyses and manifests the benefits of populated databases for a better assessment of diversity and variability of relatively unknown pathogens. Overall, genomic comparative analysis enhances the understanding of transmission and epidemiological patterns of this species and suggests vertical transmission of the pathogen, including the resistance genes, within the Spanish integrated systems.ca_ES
dc.description.sponsorshipThis study was partially funded by grant RTI2018-095586-B-C22 from the Ministerio de Economía y Competitividad (MINECO), by the CERCA program from Generalitat de Catalunya, and by the AEMPS/PRAN with European funds with reference “Mecanismo de recuperación y resiliencia.” J.G.-M. is a PhD student from the Autonomous University of Barcelona, Biotechnology Program. N.G.-E. and M.M. (FDA) were supported by the FDA Foods Program Intramural Funds.ca_ES
dc.identifier.doihttps://doi.org/10.1128/spectrum.01185-22
dc.identifier.idgrec032526
dc.identifier.issn2165-0497
dc.identifier.urihttp://hdl.handle.net/10459.1/84423
dc.language.isoengca_ES
dc.publisherAmerican Society for Microbiologyca_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095586-B-C22/ES/OPTIMIZATION OF DIAGNOSIS AND TREATMENT OF POSTWEANING DIARRHEAS IN PIGS: TOWARDS A RATIONAL USE OF ANTIBIOTICS/ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1128/spectrum.01185-22ca_ES
dc.relation.ispartofMicrobiology Spectrum, 2022, vol.10, núm. 4, p. 1-13ca_ES
dc.rightscc-by (c) Guitart-Matas et al., 2022ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectActinobacillusca_ES
dc.subjectSwineca_ES
dc.subjectWhole genome sequencingca_ES
dc.titleRevealing genomic insights of the unexplored porcine pathogen actinobacillus pleuropneumoniae using whole genome sequencingca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_ES
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
032526.pdf
Size:
2.25 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: