Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors

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dc.contributor.authorGarcía, María J.
dc.contributor.authorPalma-Bautista, Candelario
dc.contributor.authorVázquez-García, José G.
dc.contributor.authorRojano-Delgado, Antonia M.
dc.contributor.authorOsuna, Maria D.
dc.contributor.authorTorra Farré, Joel
dc.contributor.authorPrado, Rafael de
dc.date.accessioned2020-10-27T11:26:30Z
dc.date.available2020-10-27T11:26:30Z
dc.date.issued2019-10
dc.date.updated2020-10-27T11:26:30Z
dc.description.abstractAmaranthus hybridus is one of the main weed species in Córdoba, Argentina. Until recently, this weed was effectively controlled with recurrent use of glyphosate. However, a population exhibiting multiple resistance (MR2) to glyphosate and imazamox appeared in a glyphosate resistant (GR) soybean field, with levels of resistance up to 93 and 38-fold higher to glyphosate and imazamox, respectively compared to the susceptible (S) population. In addition to imidazolinones, MR2 plants showed high resistance levels to sulfonylamino-carbonyl (thio) benzoates and moderate resistance to sulfonylureas and triazolopyrimidines. Multiple amino acid substitutions were found in both target genes, acetolactate synthase (ALS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), responsible for conferring high herbicides resistance levels in this A. hybridus population. In the case of EPSPS, the triple amino acid substitution TAP-IVS was found. In addition, MR2 plants also showed increased EPSPS gene expression compared to susceptible plants. A Ser653Asn substitution was found in the ALS sequence of MR2, explaining the pattern of cross-resistance to the ALS-inhibitor herbicide families found at the ALS enzyme activity level. No other mutations were found in other conserved domains of the ALS gene. This is the first report worldwide of the target site resistance mechanisms to glyphosate and ALS inhibitors in multiple herbicide resistance Amaranthus hybridus.
dc.description.sponsorshipThe funding was grant by Asociación de agroquímicos y Medio ambiente.
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1038/s41598-020-74430-0
dc.identifier.idgrec030524
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10459.1/69713
dc.language.isoeng
dc.publisherNature Research
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1038/s41598-020-74430-0
dc.relation.ispartofScientific Reports, 2019, vol. 10, p. 17681
dc.rightscc-by (c) García, María J. et al., 2019
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPlant breeding
dc.subjectPlant sciences
dc.titleMultiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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