Validation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snack

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dc.contributor.authorYuste, Silvia
dc.contributor.authorMacià i Puig, Ma Alba
dc.contributor.authorLudwig, Iziar A.
dc.contributor.authorRomero Fabregat, Mª Paz
dc.contributor.authorFernández Castillejo, Sara
dc.contributor.authorCatalán Santos, Úrsula
dc.contributor.authorMotilva Casado, Mª José
dc.contributor.authorRubió Piqué, Laura
dc.date.accessioned2019-02-11T11:21:18Z
dc.date.available2019-11-02T23:17:40Z
dc.date.issued2018
dc.description.abstractScope: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. Methods and results: An analytical method based on blood sampling with DBS cards combined with LC–MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (μSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-γ -valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or μSPE. Conclusions: Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.ca_ES
dc.description.sponsorshipS.Y. and A.M. contributed equally to this work. This study was sup- ported by the Spanish Ministry of Industry, Economy and Competitiveness through the AGL2016-76943-C2-1-R and AGL2016-76943-C2-2-R projects (co-funded by the European Social Fund, European Union); by the Spanish Ministry of Industry, Economy and Competitiveness through the I. A. Lud- wig postdoctoral research contract (Juan de la Cierva, FJCI-2014-20689); and by the University of Lleida through the Silvia Yuste doctoral grant. In addition, the authors are grateful to NUFRI (Mollerussa, Lleida, Cat- alonia, Spain) for providing the apples. ́ U.C. has a PERIS post-doctoral grant (SLT002/16/00239; Catalunya, Spain). NFOC-Salut group is a con- solidated research group of Generalitat de Catalunya, Spain (2017 SGR 522ca_ES
dc.identifier.doihttps://doi.org/10.1002/mnfr.201800623
dc.identifier.idgrec027551
dc.identifier.issn1613-4125
dc.identifier.urihttp://hdl.handle.net/10459.1/65724
dc.language.isoengca_ES
dc.publisherWileyca_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76943-C2-1-R/ES/ca_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76943-C2-2-R/ES/ca_ES
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1002/mnfr.201800623ca_ES
dc.relation.ispartofMolecular Nutrition & Food Research, 2018, vol. 62, núm. 23, e1800623ca_ES
dc.rights(c) WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 2018ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.subjectMS/MSca_ES
dc.subjectDried blood spot cardsca_ES
dc.subjectPhenolic metabolitesca_ES
dc.subjectPlasmaca_ES
dc.titleValidation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snackca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersionca_ES
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