Role of Pir1 in the construction of the Candida albicans cell wall

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dc.contributor.authorMartínez, Ana Isabel
dc.contributor.authorCastillo, Luis
dc.contributor.authorGarcera, Ana
dc.contributor.authorElorza, M. Victoria
dc.contributor.authorValentín, Eulogio
dc.contributor.authorSentandreu, Rafael
dc.date.accessioned2016-11-09T19:23:17Z
dc.date.available2016-11-09T19:23:17Z
dc.date.embargoEndDate2025-01-01
dc.date.issued2004-10
dc.date.updated2016-11-09T19:23:17Z
dc.description.abstractSearches in a Candida albicans database (http://genolist.pasteur.fr/CandidaDB/) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42% and 45% homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene (CaPIR1) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O-glycosylated and has an N-mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells and are hypersensitive to drugs that affect cell wall assembly. CaPir1 was labelled with the V5 epitope and found linked to the 1,3-b-glucan of the C. albicans wall and also by disulphide bridges when expressed in S. cerevisiae.
dc.description.sponsorshipThis work was partially supported by grants from the European Union (MRTN-CT-2003-504148), the Spanish Ministerio de Ciencia y Tecnología (BMC2003-01023) and Agència Valenciana de Ciència i Tecnologia de la Generalitat Valenciana (Grupos 03/187)
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1099/mic.0.27220-0
dc.identifier.idgrec024765
dc.identifier.issn1350-0872
dc.identifier.urihttp://hdl.handle.net/10459.1/58445
dc.language.isoeng
dc.publisherMicrobiology Society
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1099/mic.0.27220-0
dc.relation.ispartofMicrobiology-UK, 2004, num. 150, p. 3151-3161
dc.rights(c) SGM, 2004
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.classificationCandida albicans
dc.subject.classificationSaccharomyces cerevisiae
dc.subject.otherCandida albicans
dc.subject.otherSaccharomyces cerevisiae
dc.titleRole of Pir1 in the construction of the Candida albicans cell wall
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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