Epicatechin induces NF-kB, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells
| dc.contributor.author | Granado-Serrano, Ana Belén | |
| dc.contributor.author | Martín, María Angeles | |
| dc.contributor.author | Haegeman, Guy | |
| dc.contributor.author | Goya, Luis | |
| dc.contributor.author | Bravo, Laura | |
| dc.contributor.author | Ramos, Sonia | |
| dc.date.accessioned | 2018-10-16T09:16:32Z | |
| dc.date.available | 2018-10-16T09:16:32Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | The dietary flavonoid epicatechin has been reported to exhibit a wide range of biological activities. The objective of the present study was to investigate the time-dependent regulation by epicatechin on the activity of the main transcription factors (NF-kB, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor (Nrf2)) related to antioxidant defence and survival and proliferation pathways in HepG2 cells. Treatment of cells with 10mM-epicatechin induced the NF-kB pathway in a time-dependent manner characterised by increased levels of IkB kinase (IKK) and phosphorylated inhibitor of kB subunit-a (p-IkBa) and proteolytic degradation of IkB, which was consistent with an up-regulation of the NF-kB-binding activity. Time-dependent activation of the AP-1 pathway, in concert with enhanced c-Jun nuclear levels and induction of Nrf2 translocation and phosphorylation were also demonstrated. Additionally, epicatechin-induced NF-kB and Nrf2 were connected to reactive oxygen species intracellular levels and to the activation of cell survival and proliferation pathways, being phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) associated to Nrf2 modulation and ERK to NF-kB induction. These data suggest that the epicatechin-induced survival effect occurs by the induction of redox-sensitive transcription factors through a tight regulation of survival and proliferation pathways. | ca_ES |
| dc.description.sponsorship | The present study was supported by the grants AGL2004-302, AGL2007-64042 and CSD2007-00063 from the Spanish Ministry of Science and Innovation (CICYT) and 200870 I198 (CSIC). A. B. G.-S. is a predoctoral fellow of the Spanish Ministry of Science and Education. | ca_ES |
| dc.identifier.doi | https://doi.org/10.1017/S0007114509991747 | |
| dc.identifier.idgrec | 018373 | |
| dc.identifier.issn | 0007-1145 | |
| dc.identifier.uri | http://hdl.handle.net/10459.1/64884 | |
| dc.language.iso | eng | ca_ES |
| dc.publisher | Cambridge University Press | ca_ES |
| dc.relation | info:eu-repo/grantAgreement/MEC//AGL2004-302/ES/ | |
| dc.relation | info:eu-repo/grantAgreement/MEC//AGL2007-64042/ES/EVALUACION IN VITRO E IN VIVO DEL POTENCIAL QUIMIOPREVENTIVO DE FLAVANOLES DE LA DIETA FRENTE AL DESARROLLO DE CANCER COLONICO/ | |
| dc.relation | info:eu-repo/grantAgreement/MEC//CSD2007-00063/ES/Nuevos Ingredientes de Alimentos Funcionales para Mejorar la Salud/ | |
| dc.relation.isformatof | Reproducció del document publicat a https://doi.org/10.1017/S0007114509991747 | ca_ES |
| dc.relation.ispartof | British Journal of Nutrition, 2010, vol. 103, p. 168–179 | ca_ES |
| dc.rights | (c) Cambridge University Press, 2010 | ca_ES |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_ES |
| dc.subject | Epicatechin | ca_ES |
| dc.subject | NF-kB signalling | ca_ES |
| dc.subject | Activator protein-1 | ca_ES |
| dc.subject | Nuclear factor-erythroid 2p45-related factor-2 | ca_ES |
| dc.title | Epicatechin induces NF-kB, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells | ca_ES |
| dc.type | info:eu-repo/semantics/article | ca_ES |
| dc.type.version | info:eu-repo/semantics/publishedVersion | ca_ES |