Value-added compounds were obtained from pine wood bio-oil, converting it into a renewable source for several chemicals: acids (acetic acid), sugars (levoglucosan), furan derivatives (furfural) and phenolics (catechol, phenol, 4-propylguaiacol, vanillin). To achieve this, firstly a comparison of a direct pyrolysis at 500 ◦C versus a twostaged pyrolysis (300 ◦C and 500 ◦C) was made. It was determined that a two-staged pyrolysis ensue in the obtainment of less complex liquid fractions since it concentrates families of compounds in different phases of biooil, facilitating their subsequent separation. Secondly, two methodologies (A and B) were designed to establish a procedure for separating the various chemical groups present in bio-oil. Methodology A consisted of a first vacuum distillation followed by an extraction process that combined an alkaline extraction and a simple extraction with ethyl acetate. Methodology B consisted of a first extraction with trioctylamine and ethyl acetate followed by a vacuum distillation and an extraction with hot water. The results showed that methodology A was better for the separation of chemical compounds from bio-oil, obtaining one fraction with the carboxylic acid content and furan derivatives, a second fraction containing the phenolic compounds and a third fraction enriched with the sugar content.