The introduction and spread of the Neolithic “way of life” in Europe was a process that took several millennia, followed by different rhythms and displayed singularities in each geographic area. It was therefore a very complex phenomenon that, despite highly significant advances in research in recent decades, is yet to be fully understood. To deepen our understanding of the very early stages of the introduction of herding and agriculture throughout the Old Continent, the 1st Conference on the Early Neolithic of Europe was organised in Barcelona on 6–8 November 2019. The conference was a great success with more than 200 participants, creating a stimulating arena to discuss and debate, exclusively, the transition to the Neolithic in Europe. This special issue brings together 52 of the contributions presented in Barcelona, offering an interesting overview of the current state of research across Europe, from the Anatolia to the Algarve, highlighting the geographical, chronological and socioeconomic diversity of the transformation processes involved in the Neolithisation of Europe and providing useful starting points for future research.