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dc.contributor.authorSaba-Mayoral, Andrea
dc.contributor.authorBassie Rene, Ludovic
dc.contributor.authorCapell Capell, Teresa
dc.date.accessioned2022-04-29T09:29:37Z
dc.date.available2022-04-29T09:29:37Z
dc.date.issued2022
dc.identifier.issn0962-8819
dc.identifier.urihttp://hdl.handle.net/10459.1/83183
dc.description.abstractWe report the development of an efficient and reproducible genetic transformation system for the recalcitrant Spanish elite rice paella genotype, Bomba. Preconditioned embryos derived from dry seeds were bombarded with gold particles carrying a plasmid containing a screenable and a selectable marker. We confirmed integration and expression of hpt and gusA in the rice genome. Transformation frequency was ca: 10% in several independent experiments. We show Mendelian inheritance of the input transgenes and zygosity determination of the transgenic lines in the T1 generation. A unique and critical step for the regeneration of plants from transformed tissue was shading during the early stages of regeneration, combined with a specific cytokinin:auxin ration at the onset of shifting callus to regeneration media.ca_ES
dc.description.sponsorshipOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.ca_ES
dc.language.isoengca_ES
dc.publisherSPRINGERca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1007/s11248-022-00303-zca_ES
dc.relation.ispartofTransgenic research, 2022, p.1-16ca_ES
dc.rightscc-by (c) Saba et al., 2022ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDirect DNA transferca_ES
dc.subjectGenetic transformationca_ES
dc.subjectOryza sativaca_ES
dc.subjectZygosity determinationca_ES
dc.subject.otherArròs--Genèticaca_ES
dc.subject.otherArròs--Varietatsca_ES
dc.titleDevelopment of a facile genetic transformation system for the Spanish elite rice paella genotype Bombaca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.identifier.idgrec032227
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s11248-022-00303-z


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cc-by (c) Saba et al., 2022
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