Serum lysophospholipidome of dietary origin as a suitable susceptibility/risk biomarker of human hypercholesterolemia: a cross-sectional study

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2022Author
Calderón-Pérez, Lorena
Suárez-García, Susana
Pedret, Anna
Suárez, Manuel
Llauradó, Elisabet
Bas, Josep M. del
Caimari, Antoni
Puiggrós, Francesc
Arola, Lluís
Solà, Rosa
Valls, Rosa M.
Suggested citation
Calderón-Pérez, Lorena;
Suárez-García, Susana;
Pedret, Anna;
Suárez, Manuel;
Llauradó, Elisabet;
Rubió Piqué, Laura;
...
Valls, Rosa M..
(2022)
.
Serum lysophospholipidome of dietary origin as a suitable susceptibility/risk biomarker of human hypercholesterolemia: a cross-sectional study.
Clinical nutrition, 2022, vol. 41, núm. 2, p.489-499.
https://doi.org/10.1016/j.clnu.2021.11.033.
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Background & aims: Whether bioactive lysophospholipids (lyso-PLs) and trimethylamine-N-oxide
(TMAO) serve as non-invasive biomarkers in early human hypercholesterolemia (HC) is unknown. This
study aimed to assess whether serum lyso-PLs and plasma TMAO may be suitable susceptibility/risk
biomarkers of HC in humans. Secondarily, we aimed to evaluate the relationships between targeted
metabolites, diet composition and circulating liver transaminases, and verify these results in hamsters.
Methods: A targeted metabolomics and lipidomics approach determined plasma TMAO and serum
lysophosphatidylcholines (lyso-PCs) and lysophosphatidylethanolamines (lyso-PEs) in low (L-LDL-c) and
moderate to high (MH-LDL-c) LDL-cholesterol subjects. Additionally, the relationships between targeted
metabolites, liver transaminases and diet, particularly fatty acid intake, were tested. In parallel, plasma
and liver lyso-PL profiles were studied in 16 hamsters fed a moderate high-fat (HFD) or low-fat (LFD) diet
for 30 days.
Results: Predictive models identified lyso-PC15:0 and lyso-PE18:2 as the most discriminant lyso-PLs
among groups. In MH-LDL-c (n ¼ 48), LDL-cholesterol and saturated FAs were positively associated
with lyso-PC15:0, whereas in L-LDL-c (n ¼ 70), LDL-cholesterol and polyunsaturated fatty acids (PUFAs)
were negatively and positively related to lyso-PE18:2, respectively. Interestingly, in MH-LDL-c, the lower
lyso-PE 18:2 concentrations were indicative of higher LDL-cholesterol levels. Intrahepatic accumulation
of lyso-PLs-containing essential n-6 PUFAs, including lyso-PE18:2, were higher in HFD-fed hamsters than
LFD-fed hamsters.
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Clinical nutrition, 2022, vol. 41, núm. 2, p.489-499European research projects
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