Genome-wide identification and characterization of bZIP transcription factors and their expression profile under abiotic stresses in Chinese pear (Pyrus bretschneideri)
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Date
2021
Authors
Aamir Manzoor, Muhammad
Mudassar Manzoor, Muhammad
Li, Guohui
Abdullah, Muhammad
Han, Wang
Wenlong, Han
Shakoor, Awais
Waheed Riaz, Muhammad
Rehman, Shamsur
Cai, Yongping
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Abstract
Background: In plants, basic leucine zipper transcription factors (TFs) play important roles in multiple biological
processes such as anthesis, fruit growth & development and stress responses. However, systematic investigation and
characterization of bZIP-TFs remain unclear in Chinese white pear. Chinese white pear is a fruit crop that has
important nutritional and medicinal values.
Results: In this study, 62 bZIP genes were comprehensively identified from Chinese Pear, and 54 genes were
distributed among 17 chromosomes. Frequent whole-genome duplication (WGD) and dispersed duplication (DSD)
were the major driving forces underlying the bZIP gene family in Chinese white pear. bZIP-TFs are classified into 13
subfamilies according to the phylogenetic tree. Subsequently, purifying selection plays an important role in the
evolution process of PbbZIPs. Synteny analysis of bZIP genes revealed that 196 orthologous gene pairs were
identified between Pyrus bretschneideri, Fragaria vesca, Prunus mume, and Prunus persica. Moreover, cis-elements that
respond to various stresses and hormones were found on the promoter regions of PbbZIP, which were induced by
stimuli. Gene structure (intron/exon) and different compositions of motifs revealed that functional divergence
among subfamilies. Expression pattern of PbbZIP genes differential expressed under hormonal treatment abscisic
acid, salicylic acid, and methyl jasmonate in pear fruits by real-time qRT-PCR.
Conclusions: Collectively, a systematic analysis of gene structure, motif composition, subcellular localization,
synteny analysis, and calculation of synonymous (Ks) and non-synonymous (Ka) was performed in Chinese white
pear. Sixty-two bZIP-TFs in Chinese pear were identified, and their expression profiles were comprehensively
analyzed under ABA, SA, and MeJa hormones, which respond to multiple abiotic stresses and fruit growth and
development. PbbZIP gene occurred through Whole-genome duplication and dispersed duplication events. These
results provide a basic framework for further elucidating the biological function characterizations under multiple
developmental stages and abiotic stress responses.
Citation
Journal or Serie
BMC Plant Biology, 2021, vol. 21, núm. 1, p. 1-16