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dc.contributor.authorJové Font, Mariona
dc.contributor.authorCabré, Rosanna
dc.contributor.authorMota Martorell, Natàlia
dc.contributor.authorMartin Garí, Meritxell
dc.contributor.authorObis, Èlia
dc.contributor.authorRamos, Paula
dc.contributor.authorCanales , Iván
dc.contributor.authorGalo-Licona, José Daniel
dc.contributor.authorSol, Joaquim
dc.contributor.authorNogueras Penabad, Lara
dc.contributor.authorTorres Cabestany, Pascual
dc.contributor.authorPortero Otín, Manuel
dc.contributor.authorAyala Jové, Ma. Victoria (Maria Victoria)
dc.contributor.authorFerrer, Isidre
dc.contributor.authorPamplona Gras, Reinald
dc.description.abstractLipids are closely associated with brain structure and function. However, the potential changes in the lipidome induced by aging remain to be elucidated. In this study, we used chromatographic techniques and a mass spectrometry-based approach to evaluate age-associated changes in the lipidome of the frontal cortex and cerebellum obtained from adult male Wistar rats (8 months), aged male Wistar rats (26 months), and aged male Wistar rats submitted to a methionine restriction diet (MetR) as an anti-aging intervention for 8 weeks. The outcomes revealed that only small changes (about 10%) were observed in the lipidome profile in the cerebellum and frontal cortex during aging, and these changes differed, in some cases, between regions. Furthermore, a MetR diet partially reversed the effects of the aging process. Remarkably, the most affected lipid classes were ether-triacylglycerols, diacylglycerols, phosphatidylethanolamine N-methylated, plasmalogens, ceramides, and cholesterol esters. When the fatty acid profile was analyzed, we observed that the frontal cortex is highly preserved during aging and maintained under MetR, whereas in the cerebellum minor changes (increased monounsaturated and decreased polyunsaturated contents) were observed and not reversed by MetR. We conclude that the rat cerebellum and frontal cortex have efficient mechanisms to preserve the lipid profile of their cell membranes throughout their adult lifespan in order to maintain brain structure and function. A part of the small changes that take place during aging can be reversed with a MetR diet applied in old age.
dc.description.sponsorshipThis research was funded by the Spanish Ministry of Science, Innovation, and Universities (grant RTI2018-099200-B-I00), and the Generalitat of Catalonia: Agency for Management of University and Research Grants (2017SGR696) to R.P.; and from Spanish Ministry of Economy and Competitiveness, Institute of Health Carlos III (PI20-0155) to M.P.-O. This study was co-financed by FEDER funds from the European Union (“A way to build Europe”). IRBLleida is a CERCA Programme/Generalitat of Catalonia.
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofInternational Journal Of Molecular Sciences, 2021, vol. 22, núm. 22, p. 12517
dc.rightscc-by (c) authors, 2021
dc.subject(Lip)oxidation-derived protein damage markers
dc.subjectMass spectrometry
dc.subjectMethionine restriction
dc.subjectSterol lipids
dc.titleAge-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age

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