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dc.contributor.authorGuerrero-Gómez, David
dc.contributor.authorMora-Lorca, José Antonio
dc.contributor.authorSáenz-Narciso, Beatriz
dc.contributor.authorNaranjo-Galindo, Francisco José
dc.contributor.authorMuñoz-Lobato, Fernando
dc.contributor.authorParrado-Fernández, Cristina
dc.contributor.authorGoikolea, Julen
dc.contributor.authorCedazo-Minguez, Ángel
dc.contributor.authorLink, Christopher D.
dc.contributor.authorNeri, Christian
dc.contributor.authorSequedo, María Dolores
dc.contributor.authorVázquez-Manrique, Rafael P.
dc.contributor.authorFernández-Suárez, Elena
dc.contributor.authorGoder, Veit
dc.contributor.authorPane Domenec, Roser
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorAskjaer, Peter
dc.contributor.authorCabello, Juan
dc.contributor.authorMiranda-Vizuete, Antonio
dc.date.accessioned2022-01-11T13:37:14Z
dc.date.available2022-01-11T13:37:14Z
dc.date.issued2019
dc.identifier.issn1350-9047 (paper)
dc.identifier.issn1476-5403 (electrònic)
dc.identifier.urihttp://hdl.handle.net/10459.1/72704
dc.description.abstractIn the presence of aggregation-prone proteins, the cytosol and endoplasmic reticulum (ER) undergo a dramatic shift in their respective redox status, with the cytosol becoming more oxidized and the ER more reducing. However, whether and how changes in the cellular redox status may affect protein aggregation is unknown. Here, we show that C. elegans loss-of-function mutants for the glutathione reductase gsr-1 gene enhance the deleterious phenotypes of heterologous human, as well as endogenous worm aggregation-prone proteins. These effects are phenocopied by the GSH-depleting agent diethyl maleate. Additionally, gsr-1 mutants abolish the nuclear translocation of HLH-30/TFEB transcription factor, a key inducer of autophagy, and strongly impair the degradation of the autophagy substrate p62/SQST-1::GFP, revealing glutathione reductase may have a role in the clearance of protein aggregates by autophagy. Blocking autophagy in gsr-1 worms expressing aggregation-prone proteins results in strong synthetic developmental phenotypes and lethality, supporting the physiological importance of glutathione reductase in the regulation of misfolded protein clearance. Furthermore, impairing redox homeostasis in both yeast and mammalian cells induces toxicity phenotypes associated with protein aggregation. Together, our data reveal that glutathione redox homeostasis may be central to proteostasis maintenance through autophagy regulation.ca_ES
dc.description.sponsorship. The Spanish Ministry of Economy and Competitiveness supported EF-S and VG (BFU2016–78265-P), PA (BFU2016– 79313-P and MDM-2016–0687), and AM-V (BFU2015–64408-P). AM-V was also supported by the Instituto de Salud Carlos III (PI11/ 00072) and RPV-M (CPII16/00004, PI14/00949 and PI17/00011). All projects were cofinanced by the Fondo Social Europeo (FEDER). AM-V is a member of the GENIE and EU-ROS Cost Actions of the European Union and RPV-M is a Marie Curie Fellow (CIG322034, EU).ca_ES
dc.language.isoengca_ES
dc.publisherSpringer Natureca_ES
dc.relationMINECO/PN2013-2016/BFU2016–78265-Pca_ES
dc.relationMINECO/PN2013-2016/BFU2016–79313-Pca_ES
dc.relationMINECO/PN2013-2016/BFU2015-64408-Pca_ES
dc.relation.isformatofVersió postprint del document publicat a https://doi.org/10.1038/s41418-018-0270-9ca_ES
dc.relation.ispartofCell Death and Differentiation, 2009, vol. 26, núm. 9, p. 1545-1565ca_ES
dc.rights(c) ADMC Associazione Differenziamento e Morte Cellulare, 2019ca_ES
dc.subjectAutophagy
dc.subjectCaenorhabditis elegans
dc.subjectCell blebbing
dc.subjectDiethyl maleate
dc.titleLoss of glutathione redox homeostasis impairs proteostasis by inhibiting autophagy-dependent protein degradationca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.identifier.idgrec028586
dc.type.versioninfo:eu-repo/semantics/acceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1038/s41418-018-0270-9


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