Various studies have analysed differences in the spatial behaviour of tourists based on gender. However, the majority of them have been based on methods that collected data from informants. The present research assesses the feasibility of using Instagram as a tool for identifying these differences. Based on user-generated content, it uses cartography to analyse differences in tourist behaviour, segmenting tourists according to gender and whether they travelled alone or with other people. The work analyses 1,114 photographs taken by 100 Instagram users (50 men and 50 women) who visited the city of Barcelona to observe differences in tourist behaviour. This is something that has hitherto received little academic attention but which is fundamental if we are to improve our knowledge of the influence of gender on the spaces that toursits visit. The results of this Instagram analysis highlight spatially differentiated behaviour. This research opens the door to using Instagram to identify different gender-related forms of tourist behaviour related to the places and themes that tourists photograph.