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dc.contributor.authorMaiques Carlos, Oscar
dc.contributor.authorCuevas, Dolors
dc.contributor.authorGarcía Dios, Diego Andrés
dc.contributor.authorCoenegrachts, Lieve
dc.contributor.authorSantacana Espasa, Maria
dc.contributor.authorVelasco, Ana
dc.contributor.authorRomero, Marta
dc.contributor.authorGatius Calderó, Sònia
dc.contributor.authorLambrechts, Diether
dc.contributor.authorMüller, Sven
dc.contributor.authorPedersen, Hans Christian
dc.contributor.authorDolcet Roca, Xavier
dc.contributor.authorAmant, Frederic
dc.contributor.authorMatias-Guiu, Xavier
dc.description.abstractAims: To check the usefulness of a standardized protocol of PTEN FISH in 31 endometrial carcinomas (ECs) in comparison with SNP array (SNPA), multiplex ligation-dependent probe amplification (MLPA), and immunohistochemistry. Methods and results: Fluorescence in-situ hybridization analysis showed two PTEN copies in 17 cases, three copies in nine cases, hemizygous deletion in two cases, and diverse cell populations with different PTEN copy number in three cases. A good correlation was seen between FISH and SNPA, particularly in cases with three copies. FISH identified two cases with entire deletion of chromosome 10, but did not identify a focal deletion of PTEN. Five cases with PTEN deletion and duplication of the second allele by SNPA were interpreted as normal by FISH. Concordance between FISH and MLPA was seen in 15 cases with two copies, and in two cases with PTEN deletion. Six cases were interpreted as amplified by MLPA, but showed polyploidy by FISH. FISH was superior to SNPA and MLPA in assessing the tumours with diverse cell populations with different PTEN copies. Conclusions: The results show good concordance between FISH, SNPA and MLPA. SNPA was superior in tumours with deletion of one copy and duplication of the second allele. FISH was superior in assessing tumour heterogeneity.ca_ES
dc.description.sponsorshipThe study was supported by a research agreement with Dako, Denmark. The research team was also supported by grants FIS PI100922, Fundacion Mutua Madrilena AP75732010, 2009SGR794, RD12/0036/ ~ 0013, Fundacion Asociaci on Espa nola contra el Can- ~ cer, Programa de Intensificacion de la Investigaci on, Instituto Carlos III, Verelst Baarmoederkankerfonds, Leuven, and ENITEC (European Network for Individualized Treatment of Endometrial Carcinoma). F. Amant is senior researcher for the research fund Flandersb (FWO). Tumour samples were obtained with the support of Xarxa Catalana de Bancs de Tumours, and Plataforma de Biobancos ISCIII (PT13/ 0010/0014).ca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofHistopathology, 2014, vol. 65, núm. 3, p. 371-388ca_ES
dc.rightscc-by-nc (c) Wiley, 2014ca_ES
dc.subjectFluorescence in-situ hybridizationca_ES
dc.subjectPreanalytical variablesca_ES
dc.titleFISH analysis of PTEN in endometrial carcinoma. Comparison with SNP arrays and MLPAca_ES

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cc-by-nc (c) Wiley, 2014
Except where otherwise noted, this item's license is described as cc-by-nc (c) Wiley, 2014