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dc.contributor.authorAldecoa, Iban
dc.contributor.authorAtares, Begoña
dc.contributor.authorTarragona Foradada, Jordi
dc.contributor.authorBernet, Laia
dc.contributor.authorSardon, Jose Domingo
dc.contributor.authorPereda, Teresa
dc.contributor.authorVillar, Carlos
dc.contributor.authorMendez, M. Carmen
dc.contributor.authorGonzalez-Obeso, Elvira
dc.contributor.authorElorriaga, Kepa
dc.contributor.authorLopez Alonso, Guadalupe
dc.contributor.authorZamora, Javier
dc.contributor.authorPlanell, Nuria
dc.contributor.authorPalacios, José
dc.contributor.authorCastells, Antoni
dc.contributor.authorMatias-Guiu, Xavier
dc.contributor.authorCuatrecasas, Miriam
dc.description.abstractStage I–II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated with an increased risk of disease recurrence and poor survival. This prospective multicentre study aimed to determine the relationship between LN molecular tumour burden and conventional high-risk factors in stage I–II colon cancer patients. A total of 1940 LN from 149 pathologically assessed pN0 colon cancer patients were analysed for the amount of tumour cytokeratin 19 (CK19) messenger RNA (mRNA) with the quantitative reverse transcription loop-mediated isothermal amplification molecular assay One-Step Nucleic Acid Amplification. Patient’s total tumour load (TTL) resulted from the sum of all CK19 mRNA tumour copies/μL of each positive LN from the colectomy specimen. A median of 15 LN were procured per case (IQR 12;20). Molecular positivity correlated with high-grade (p < 0.01), mucinous/signet ring type (p = 0.017), male gender (p = 0.02), number of collected LN (p = 0.012) and total LN weight per case (p < 0.01). The TTL was related to pT stage (p = 0.01) and tumour size (p < 0.01) in low-grade tumours. Multivariate logistic regression showed independent correlation of molecular positivity with gender, tumour grade and number of fresh LN [AUC = 0.71 (95 % CI = 0.62–0.79)]. Our results show that lymph node CK19 mRNA detection correlates with classical high-risk factors in stage I–II colon cancer patients. Total tumour load is a quantitative and objective measure that may help to better stage early colon cancer patients.ca_ES
dc.description.sponsorshipWork supported by the Banc de Tumors-Biobanc Hospital Clinic-IDIBAPS and Xarxa de Bancs de Tumors de Catalunya (XBTC), and by grants from the Fundación Científica de la Asociación Española Contra el Cáncer (GCB13131592CAST), Ministerio de Economía y Competitividad (SAF2014–54,453-R), Agència de Gestió d’Ajuts Universitaris i de Recerca (2014SGR135), and by Sysmex Coorp Spain (Sant Just Desvern, Spain). CIBERehd is funded by the Instituto de Salud Carlos IIIca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofVirchows Archiv, 2016, vol. 469, p. 385-394ca_ES
dc.rightscc-by (c) Aldecoa et al., 2016ca_ES
dc.subjectColorectal neoplasmsca_ES
dc.subjectNeoplasm stagingca_ES
dc.subjectMolecular pathologyca_ES
dc.subjectLymph nodesca_ES
dc.subjectCytokeratin 19ca_ES
dc.titleMolecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective studyca_ES

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