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dc.contributor.authorRobles-Jimenez, Lizbeth E.
dc.contributor.authorMendez Martinez, Ivan
dc.contributor.authorBalcells Terés, Joaquim
dc.contributor.authorGonzález Ronquillo, Manuel
dc.date.accessioned2021-03-16T11:18:31Z
dc.date.issued2020
dc.identifier.isbn9789811433801
dc.identifier.isbn9789811433825
dc.identifier.urihttp://hdl.handle.net/10459.1/70755
dc.description.abstractThe importance of quantifying ruminal microbial crude protein synthesis has promoted the development and comparison of several different methods for precise determination of both the amount and rate of synthesis. One major challenge is in estimating and differentiating protein in the rumen between microbial, dietary, and endogenous fractions, and to correctly isolate the solid and liquid microbial fraction of the rumen contents. This is further complicated by the goal of using non-invasive methods as much as is feasible, such as avoiding the use of fistulated animals; the selection of an appropriate microbial marker, specifically one that behaves similarly in the solid-associated and liquid-associated microbial fractions. It is also vital to be able to accurately estimate the contribution of microbial protein to overall nitrogen used by the animal, which can be accomplished by the use of 15N labeled, as assimilated by ruminal bacteria, and by the quantification of labeled nitrogen via mass spectrometry (15N/14N). This review focuses on challenges regarding accurate quantification of microbial crude protein synthesis in the rumen, as well as providing the methodology for quantification using the 15N marker. This review is based on the collection of scientific papers from the main research groups in feed and animal nutrition in ruminants.ca_ES
dc.language.isoengca_ES
dc.publisherBentham Science Publishersca_ES
dc.relation.isformatofVersió postprint del document publicat a: http://dx.doi.org/10.2174/97898114338251200101ca_ES
dc.relation.ispartofApplications of Modern Mass Spectrometry / editors: Atta-ur-Rahman , M. Iqbal Choudhary, Syed Ghulam Musharraf, vol. 1, p. 1-23ca_ES
dc.rights(c) Bentham Science Publishers, 2020ca_ES
dc.subjectMicrobial proteinca_ES
dc.subjectEndogenous excretionca_ES
dc.subjectRuminantsca_ES
dc.subjectPurine derivativesca_ES
dc.titleApplications of mass spectrometry for the determination of the microbial crude protein synthesis in ruminantsca_ES
dc.typeinfo:eu-repo/semantics/bookPartca_ES
dc.identifier.idgrec024451
dc.type.versioninfo:eu-repo/semantics/acceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccessca_ES
dc.identifier.doihttp://dx.doi.org/10.2174/97898114338251200101
dc.date.embargoEndDate2022-12-31


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