Applications of mass spectrometry for the determination of the microbial crude protein synthesis in ruminants
Fecha de publicación2020
Robles-Jimenez, Lizbeth E.
Mendez Martinez, Ivan
González Ronquillo, Manuel
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The importance of quantifying ruminal microbial crude protein synthesis has promoted the development and comparison of several different methods for precise determination of both the amount and rate of synthesis. One major challenge is in estimating and differentiating protein in the rumen between microbial, dietary, and endogenous fractions, and to correctly isolate the solid and liquid microbial fraction of the rumen contents. This is further complicated by the goal of using non-invasive methods as much as is feasible, such as avoiding the use of fistulated animals; the selection of an appropriate microbial marker, specifically one that behaves similarly in the solid-associated and liquid-associated microbial fractions. It is also vital to be able to accurately estimate the contribution of microbial protein to overall nitrogen used by the animal, which can be accomplished by the use of 15N labeled, as assimilated by ruminal bacteria, and by the quantification of labeled nitrogen via mass spectrometry (15N/14N). This review focuses on challenges regarding accurate quantification of microbial crude protein synthesis in the rumen, as well as providing the methodology for quantification using the 15N marker. This review is based on the collection of scientific papers from the main research groups in feed and animal nutrition in ruminants.
Es parte deApplications of Modern Mass Spectrometry / editors: Atta-ur-Rahman , M. Iqbal Choudhary, Syed Ghulam Musharraf, vol. 1, p. 1-23
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Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius) Guerouali, Abdelhai; El Gass, Youssef; Balcells Terés, Joaquim; Belenguer, Álvaro; Nolan, John (Cambridge University Press, 2004)Five experiments were carried out to extend knowledge of purine metabolism in the camel (Camelus dromedarius) and to establish a model to enable microbial protein outflow from the forestomachs to be estimated from the ...
Urinary excretion of purine derivatives as an index of microbial-nitrogen intake in growing rabbits Balcells Terés, Joaquim; Ganuza, J. M.; Pérez, J. F.; Martin-Orúe, S. M.; González Ronquillo, Manuel (Cambridge University Press, 1998)Three experiments were carried out to establish a response model between intake and urinary excretion of purine compounds. In Expt 1 the relationship between the intake of purine bases (PB) and the excretion of total ...
Effects of crude protein level in the concentrate and time allotment on pasture on milk yield, urinary nitrogen, and purine derivative excretion in lactating Latxa ewes Fernández, R.; Seradj, Ahmad Reza; Oregi, L. M.; García-Rodríguez, A.; Balcells Terés, Joaquim (CSIRO Publishing, 2015)This study assessed the influence of reducing the crude protein (CP) content (from 190 to 130 g/kg CP/kg) in the supplementary concentrate and time allotment on pasture (TAP) on the milk yield, bodyweight, and the urinary ...