Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity

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2020-07-01Author
Pradas Barriga, Irene
Sanchez, Isabel
Gómez, José
Barja, Gustavo
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Mota Martorell, Natàlia;
Jové Font, Mariona;
Pradas Barriga, Irene;
Sanchez, Isabel;
Gómez, José;
Naudí i Farré, Alba;
...
Pamplona Gras, Reinald.
(2020)
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Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity.
Redox Biology, 2020, vol. 34, núm. 101539, p. 1-10.
https://doi.org/10.1016/j.redox.2020.101539.
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Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
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Redox Biology, 2020, vol. 34, núm. 101539, p. 1-10European research projects
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