Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity

Mota Martorell, Natalia; Jové Font, Mariona; Pradas Barriga, Irene; Sanchez, Isabel; Gómez, José; Naudí i Farré, Alba; Barja, Gustavo; Pamplona Gras, Reinald

doi:https://doi.org/10.1016/j.redox.2020.101539

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Issue date

2020-07-01

Author

Mota Martorell, Natalia

Jové Font, Mariona

Pradas Barriga, Irene

Sanchez, Isabel

Gómez, José

Naudí i Farré, Alba

Barja, Gustavo

Pamplona Gras, Reinald

Suggested citation

Mota Martorell, Natalia; Jové Font, Mariona; Pradas Barriga, Irene; Sanchez, Isabel; Gómez, José; Naudí i Farré, Alba; ... Pamplona Gras, Reinald. (2020) . Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity. Redox Biology, 2020, vol. 34, núm. 101539, p. 1-10. https://doi.org/10.1016/j.redox.2020.101539.

Abstract

Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.

URI

http://hdl.handle.net/10459.1/70698

DOI

https://doi.org/10.1016/j.redox.2020.101539

Is part of

Redox Biology, 2020, vol. 34, núm. 101539, p. 1-10

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Publicacions de projectes de recerca del Plan Nacional [2268]
Articles publicats (IRBLleida) [863]
Articles publicats (Medicina Experimental) [259]

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Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Mota Martorell et al., 2020