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dc.contributor.authorAdamo, Irene
dc.contributor.authorPiñuela, Yasmine
dc.contributor.authorBonet Lledos, José Antonio
dc.contributor.authorCastaño Soler, Carles
dc.contributor.authorMartínez de Aragón, Juan
dc.contributor.authorParladé Izquierdo, Xavier
dc.contributor.authorPera i Álvarez, Joan
dc.contributor.authorAlday, Josu G.
dc.date.accessioned2021-03-08T07:32:17Z
dc.date.issued2021-02-08
dc.identifier.issn1878-6146
dc.identifier.urihttp://hdl.handle.net/10459.1/70688
dc.description.abstractSoil sampling is a critical step affecting perceived fungal diversity, however sampling optimization for high-throughput-DNA sequencing studies have never been tested in Mediterranean forest ecosystems. We identified the minimum number of pooled samples needed to obtain a reliable description of fungal communities in terms of diversity and composition in three different Mediterranean forests (pine, oak, and mixed-pine-oak). Twenty soil samples were randomly selected in each of the three plots per type. Samples were pooled to obtain mixtures of 3, 6, 10, 15, 20 samples, and sequenced using Illumina MiSeq of fungal ITS2 amplicons. Pooling three soil samples in Pinus and Quercus stands provided consistent richness estimations, while at least six samples were needed in mixed-stands. ß-diversity decreased with increasing sample pools in monospecific-stands, while there was no effect of sample pool size on mixed35 stands. Soil sample pooling had no effect over species composition. We estimate that three samples would be already optimal to describe fungal richness and composition in Mediterranean pure stands, while at least six samples would be needed in mixed stands.
dc.description.sponsorshipThis work was partially supported by the Spanish Ministry of Science, Innovation and Universities, grants AGL2015-66001-C3, RTI2018-093907-B-C21 and RTI2018-099315-A-I00. I.A. was supported by a Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Cofund grant agreement No. 801596, J.G.A. was supported by Ramon y Cajal fellowship (RYC-2016-20528) and J.A.B. benefitted from a Serra-Húnter Fellowship provided by the Generalitat of Catalunya.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherBritish Mycological Society
dc.publisherElsevier
dc.relationMINECO/PN2013-2016/AGL2015-66001-C3
dc.relationMINECO/PN2017-2020/RTI2018-093907-B-C21
dc.relationMINECO/PN2017-2020/RTI2018-099315-A-I00
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.funbio.2021.01.005
dc.relation.ispartofFungal Biology, 2021, vol. 125, núm. 6, p. 469-476.
dc.rightscc-by-nc-nd (c) The British Mycological Society, 2021
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es
dc.subjectFungal communities
dc.subjectDNA metabarcoding
dc.subjectSample pooling
dc.subjectMixed forests
dc.subjectMediterranean forest
dc.subjectBeta-diversity
dc.titleSampling forest soils to describe fungal diversity and composition. Which is the optimal sampling size in Mediterranean pure and mixed pine oak forests?
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2021-03-08T07:32:18Z
dc.identifier.idgrec031079
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccess
dc.identifier.doihttps://doi.org/10.1016/j.funbio.2021.01.005
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/801586/EU/IberusTalent
dc.date.embargoEndDate2022-02-08


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cc-by-nc-nd (c) The British Mycological Society, 2021
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) The British Mycological Society, 2021