Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species
Blasco Angulo, Natividad
Beà Tàrrega, Aida
Barés Junqué, Gisel
Navaridas Fernández de Bobadilla, Raúl
MetadataShow full item record
The apoptotic nuclease EndoG is involved in mitochondrial DNA replication. Previous results suggested that, in addition to regulate cardiomyocyte hypertrophy, EndoG could be involved in cell proliferation. Here, by using in vivo and cell culture models, we investigated the role of EndoG in cell proliferation. Genetic deletion of Endog both in vivo and in cultured cells or Endog silencing in vitro induced a defect in rodent and human cell proliferation with a tendency of cells to accumulate in the G1 phase of cell cycle and increased reactive oxygen species (ROS) production. The defect in cell proliferation occurred with a decrease in the activity of the AKT/PKB-GSK-3β-Cyclin D axis and was reversed by addition of ROS scavengers. EndoG deficiency did not affect the expression of ROS detoxifying enzymes, nor the expression of the electron transport chain complexes and oxygen consumption rate. Addition of the micropeptide Humanin to EndoG-deficient cells restored AKT phosphorylation and proliferation without lowering ROS levels. Thus, our results show that EndoG is important for cell proliferation through the control of ROS and that Humanin can restore cell division in EndoG-deficient cells and counteracts the effects of ROS on AKT phosphorylation.
Is part ofRedox Biology, 2020, vol. 37, p. 101736
European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Blasco Angulo, Natividad et al., 2020
Showing items related by title, author, creator and subject.
Cardiomyocyte hypertrophy induced by Endonuclease G deficiency requires reactive oxygen radicals accumulation and is inhibitable by the micropeptide humanin. Blasco Angulo, Natividad; Cámara, Yolanda; Núñez, Estefanía; Beà Tàrrega, Aida; Barés Junqué, Gisel; Forné Izquierdo, Carles; Ruiz Meana, Marisol; Girón, Cristina; Barba, Ignasi; García Arumí, Elena; García Dorado, David; Vázquez, Jesús; Martí, Ramón; Llovera i Tomàs, Marta; Sanchis, Daniel (Elsevier, 2018-03-01)The endonuclease G gene (Endog), which codes for a mitochondrial nuclease, was identified as a determinant of cardiac hypertrophy. How ENDOG controls cardiomyocyte growth is still unknown. Thus, we aimed at finding the ...
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe Cornago Protomártir, Marta; Garcia-Alberich, C.; Blasco Angulo, Natividad; Vall-llaura Espinosa, Núria; Nàger Grifo, Mireia; Herreros Danés, Judit; Comella i Carnicé, Joan Xavier; Sanchis, Daniel; Llovera i Tomàs, Marta (Nature Publishing Group, 2014-10-02)Glioblastoma multiforme is resistant to conventional anti-tumoral treatments due to its infiltrative nature and capability of relapse; therefore, research efforts focus on characterizing gliomagenesis and identifying ...
Ubiquitination of TrkA by Nedd4-2 regulates receptor lysosomal targeting and mediates receptor signaling Georgieva, Maya V.; Pablo Llavall, Yolanda de; Sanchis, Daniel; Comella i Carnicé, Joan Xavier; Llovera i Tomàs, Marta (Wiley, 2011)The nerve growth factor receptor TrkA (tropomyosin-related kinase receptor) participates in the survival and differentiation of several neuronal populations. The C-terminal tail of TrkA contains a PPXY motif, the binding ...