Estudio de la evolución de las poblaciones metanógenas en la digestión anaerobia del purín y su relación con el aporte exógeno de carbohidratos estructurales o arqueas exógenas

Seradj, Ahmad Reza; Morazán Nuñez, Henris Jobany; Álvarez Rodríguez, Javier; Abecia, Leticia; Babot Gaspa, Daniel; Yañez-Ruiz, David R.; Balcells Terés, Joaquim

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2013

Author

Seradj, Ahmad Reza

Morazán Nuñez, Henris Jobany

Álvarez Rodríguez, Javier

Abecia, Leticia

Babot Gaspa, Daniel

Yañez-Ruiz, David R.

Balcells Terés, Joaquim

Suggested citation

Seradj, Ahmad Reza; Morazán Nuñez, Henris Jobany; Álvarez Rodríguez, Javier; Abecia, Leticia; Babot Gaspa, Daniel; Yañez-Ruiz, David R.; Balcells Terés, Joaquim; . (2013) . Estudio de la evolución de las poblaciones metanógenas en la digestión anaerobia del purín y su relación con el aporte exógeno de carbohidratos estructurales o arqueas exógenas. XV Jornadas sobre Producción Animal : 42 jornadas de estudio : 14 y 15 de mayo de 2013, Zaragoza, tomo II, p. 869-871. Asociación Interprofesional para el Desarrollo Agrario. http://hdl.handle.net/10459.1/70426.

Abstract

Three agricultural by products (CHO:wheat straw, apple pulp and sugar beet pulp) as substrate plus 2 levels of freeze-dried cow’s feces (C0-i: 5 and 10 %) as co-inoculum were assigned in quadruplicate during 56 days of in vitro fermentation. Bottles were sampled at days (D) 0, 25 and 56 to determine the evolution of total bacteria, total and hydrogenotrophic methanogens archaea. DGGE was used to study archaea biodiversity in the slurry incubations. CHO addition did not alter bacteria titres but co-i interacts with D (P<0.001), in 5% total bacteria decreased significantly (d0 to d25) but remained steady until d56 (P>0.05) whereas in 10% titres decrease constantly (P<0.05). Incubation time also reduced the titres of archaea (P<0.01). However, hydrogenotrophic population maintained titres along incubation period and increased with Co-i (P<0.05). Most of the archaeal DGGE bands were observed in all samples, indicating a common bacterial population origin and their structure showed that incubation time and Co-i can modify the clustering pattern.

URI

http://hdl.handle.net/10459.1/70426

Is part of

XV Jornadas sobre Producción Animal : 42 jornadas de estudio : 14 y 15 de mayo de 2013, Zaragoza, tomo II, p. 869-871