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dc.contributor.authorRodríguez Colman, Maria José
dc.contributor.authorRos Salvador, Joaquim
dc.contributor.authorCabiscol Català, Elisa
dc.date.accessioned2020-12-28T09:41:08Z
dc.date.available2020-12-28T09:41:08Z
dc.date.issued2020-12
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10459.1/70144
dc.description.abstractHcm1 is a member of the forkhead transcription factor family involved in segregation, spindle pole dynamics, and budding in Saccharomyces cerevisiae. Our group described the role of Hcm1 in mitochondrial biogenesis and stress resistance, and in the cellular adaptation to mitochondrial respiratory metabolism when nutrients decrease. Regulation of Hcm1 activity occurs at the protein level, subcellular localization, and transcriptional activity. Here we report that the amount of protein increased in the G1/S transition phase when the factor accumulated in the nucleus. In the G2/M phases, the Hcm1 amount decreased, and it was translocated outside the nucleus with a network-like localization. Preparation of highly purified mitochondria by a sucrose gradient density demonstrated that Hcm1 colocalized with mitochondrial markers, inducing expression of COX1, a mitochondrial encoded subunit of cytochrome oxidase, in the G2/M phases. Taken together, these results show a new localization of Hcm1 and suggest that it acts as a mitochondrial transcription factor regulating the metabolism of this organelle.
dc.description.sponsorshipThis research was funded by Ministerio de Ciencia e Innovación (Spain), grants BFU2010-17387 and CSD2007-20 Consolider Ingenio.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI
dc.relationMICINN/PN2008-2011/BFU2010-17387
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3390/ijms21249574
dc.relation.ispartofInternational Journal of Molecular Sciences, 2020, vol. 21, núm. 24, p. 9574-9585
dc.rightscc-by (c) Rodríguez Colman, Maria José et al., 2020
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es
dc.subjectForkhead transcription factor
dc.subjectHcm1
dc.subjectMitochondria
dc.subjectCell Cycle
dc.subjectBudding yeast
dc.titleMitochondrial localization of the yeast forkhead factor Hcm1
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2020-12-28T09:41:09Z
dc.identifier.idgrec030716
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.3390/ijms21249574


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cc-by (c) Rodríguez Colman, Maria José et al., 2020
Except where otherwise noted, this item's license is described as cc-by (c) Rodríguez Colman, Maria José et al., 2020