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dc.contributor.authorFibla Palazón, Joan
dc.contributor.authorOromí Farrús, Neus
dc.contributor.authorPascual Pons, Mariona
dc.contributor.authorRoyo Sánchez-Palencia, José Luis
dc.contributor.authorPalau, Antoni
dc.contributor.authorFibla, M.
dc.description.abstractObjectives: The Brown trout is a salmonid species with a high commercial value in Europe. Life history and spawning behaviour include resident (Salmo trutta m. fario) and migratory (Salmo trutta m. trutta) ecotypes. The main objective is to apply RNA-seq technology in order to obtain a reference transcriptome of two key tissues, brain and muscle, of the riverine trout Salmo trutta m. fario. Having a reference transcriptome of the resident form will complement genomic resources of salmonid species. Data description: We generate two cDNA libraries from pooled RNA samples, isolated from muscle and brain tissues of adult individuals of Salmo trutta m. fario, which were sequenced by Illumina technology. Raw reads were subjected to de-novo transcriptome assembly using Trinity, and coding regions were predicted by TransDecoder. A final set of 35,049 non-redundant ORF unigenes were annotated. Tissue differential expression analysis was evaluated by Cuffdiff. A False Discovery Rate (FDR) ≤ 0.01 was considered for significant differential expression, allowing to identify key differentially expressed unigenes. Finally, we have identified SNP variants that will be useful tools for population genomic studies.ca_ES
dc.description.sponsorshipThis study has been supported and financed by the Biodiversity Conservation Plan of ENDESA, S.A. (ENEL Group) to JF.ca_ES
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofBMC Research Notes, 2020, vol. 13, article number 503ca_ES
dc.rightscc-by, (c) Fibla Palazón, Joan et al., 2020ca_ES
dc.subjectDe novo transcriptomeca_ES
dc.subjectBrain & muscle transcriptomeca_ES
dc.subjectSNP discoveryca_ES
dc.subjectSalmo trutta m. farioca_ES
dc.titleDe novo assembly of the Brown trout (Salmo trutta m. fario) brain and muscle transcriptome: transcript annotation, tissue differential expression profile and SNP discoveryca_ES

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cc-by, (c) Fibla Palazón, Joan et al., 2020
Except where otherwise noted, this item's license is described as cc-by, (c) Fibla Palazón, Joan et al., 2020