Resequencing the Vrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike

Thumbnail
View/Open
Issue date
2018-04-12
Author
Casas Cendoya, Ana Maria
Contreras‐Moreira, Bruno
Cantalapiedra, Carlos P.
Sakuma, Shun
Gracia Gimeno, Ma. Pilar
Moralejo Vidal, Mª Angeles
Molina Cano, José Luis
Komatsuda, Takao
Igartua Arregui, Ernesto
Suggested citation
Casas Cendoya, Ana Maria; Contreras‐Moreira, Bruno; Cantalapiedra, Carlos P.; Sakuma, Shun; Gracia Gimeno, Ma. Pilar; Moralejo Vidal, Mª Angeles; ... Igartua Arregui, Ernesto. (2018) . Resequencing the Vrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike. Molecular Breeding, 2018, vol. 38, p. 51. https://doi.org/10.1007/s11032-018-0816-z.
Impact

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists.
URI
http://hdl.handle.net/10459.1/69847
DOI
https://doi.org/10.1007/s11032-018-0816-z
Is part of
Molecular Breeding, 2018, vol. 38, p. 51
Collections