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dc.contributor.authorBlasco Carmona, Alba
dc.contributor.authorGras Artells, Sílvia
dc.contributor.authorMòdol-Caballero, Guillem
dc.contributor.authorTarabal Mostazo, Olga
dc.contributor.authorCasanovas i Llorens, Anna
dc.contributor.authorPiedrafita Llorens, Lídia
dc.contributor.authorBarranco, Alejandro
dc.contributor.authorDas, Tapas
dc.contributor.authorPereira, Suzette L.
dc.contributor.authorNavarro, Xavier
dc.contributor.authorRueda, Ricardo
dc.contributor.authorEsquerda Colell, Josep
dc.contributor.authorCalderó i Pardo, Jordi
dc.date.accessioned2020-11-02T09:43:43Z
dc.date.available2020-11-02T09:43:43Z
dc.date.issued2020-07-20
dc.identifier.issn2190-5991
dc.identifier.urihttp://hdl.handle.net/10459.1/69747
dc.description.abstractBackground The cellular mechanisms underlying the age‐associated loss of muscle mass and function (sarcopenia) are poorly understood, hampering the development of effective treatment strategies. Here, we performed a detailed characterization of age‐related pathophysiological changes in the mouse neuromuscular system. Methods Young, adult, middle‐aged, and old (1, 4, 14, and 24-30 months old, respectively) C57BL/6J mice were used. Motor behavioural and electrophysiological tests and histological and immunocytochemical procedures were carried out to simultaneously analyse structural, molecular, and functional age‐related changes in distinct cellular components of the neuromuscular system. Results Ageing was not accompanied by a significant loss of spinal motoneurons (MNs), although a proportion (~15%) of them in old mice exhibited an abnormally dark appearance. Dark MNs were also observed in adult (~9%) and young (~4%) animals, suggesting that during ageing, some MNs undergo early deleterious changes, which may not lead to MN death. Old MNs were depleted of cholinergic and glutamatergic inputs (~40% and ~45%, respectively, P < 0.01), suggestive of age‐associated alterations in MN excitability. Prominent microgliosis and astrogliosis [~93% (P < 0.001) and ~100% (P < 0.0001) increase vs. adults, respectively] were found in old spinal cords, with increased density of pro‐inflammatory M1 microglia and A1 astroglia (25‐fold and 4‐fold increase, respectively, P < 0.0001). Ageing resulted in significant reductions in the nerve conduction velocity and the compound muscle action potential amplitude (~30%, P < 0.05, vs. adults) in old distal plantar muscles. Compared with adult muscles, old muscles exhibited significantly higher numbers of both denervated and polyinnervated neuromuscular junctions, changes in fibre type composition, higher proportion of fibres showing central nuclei and lipofuscin aggregates, depletion of satellite cells, and augmented expression of different molecules related to development, plasticity, and maintenance of neuromuscular junctions, including calcitonin gene‐related peptide, growth associated protein 43, agrin, fibroblast growth factor binding protein 1, and transforming growth factor‐β1. Overall, these alterations occurred at varying degrees in all the muscles analysed, with no correlation between the age‐related changes observed and myofiber type composition or muscle topography. Conclusions Our data provide a global view of age‐associated neuromuscular changes in a mouse model of ageing and help to advance understanding of contributing pathways leading to development of sarcopenia.
dc.description.sponsorshipThis work was supported by Abbott and a grant from the Ministerio de Ciencia, Innovación y Universidades cofinancedby Fondo Europeo de Desarrollo Regional (RTI2018-099278-B-I00 to J.C. and J.E.)
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherWiley
dc.relationMINECO/PN2013-2016/RTI2018-099278-B-I00
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1002/jcsm.12599
dc.relation.ispartofJournal of Cachexia Sarcopenia And Muscle, 2020, vol. 11, núm. 6, p. 1628-1660
dc.rightscc-by-nc (c) Blasco et al., 2020
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectSarcopenia
dc.subjectAgeing
dc.subjectC57BL/6J mice
dc.subjectMotoneurons
dc.subjectCentral synapses
dc.subjectGlia
dc.subjectNeuromuscular junction
dc.subjectSkeletal muscle
dc.titleMotoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2020-11-02T09:43:43Z
dc.identifier.idgrec030267
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1002/jcsm.12599


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cc-by-nc (c) Blasco et al., 2020
Except where otherwise noted, this item's license is described as cc-by-nc (c) Blasco et al., 2020