Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors
García, María J.
Vazquez-Garcia, José G.
Rojano-Delgado, Antonia M.
Osuna, Maria D.
Prado, Rafael de
MetadataShow full item record
Amaranthus hybridus is one of the main weed species in Córdoba, Argentina. Until recently, this weed was effectively controlled with recurrent use of glyphosate. However, a population exhibiting multiple resistance (MR2) to glyphosate and imazamox appeared in a glyphosate resistant (GR) soybean field, with levels of resistance up to 93 and 38-fold higher to glyphosate and imazamox, respectively compared to the susceptible (S) population. In addition to imidazolinones, MR2 plants showed high resistance levels to sulfonylamino-carbonyl (thio) benzoates and moderate resistance to sulfonylureas and triazolopyrimidines. Multiple amino acid substitutions were found in both target genes, acetolactate synthase (ALS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), responsible for conferring high herbicides resistance levels in this A. hybridus population. In the case of EPSPS, the triple amino acid substitution TAP-IVS was found. In addition, MR2 plants also showed increased EPSPS gene expression compared to susceptible plants. A Ser653Asn substitution was found in the ALS sequence of MR2, explaining the pattern of cross-resistance to the ALS-inhibitor herbicide families found at the ALS enzyme activity level. No other mutations were found in other conserved domains of the ALS gene. This is the first report worldwide of the target site resistance mechanisms to glyphosate and ALS inhibitors in multiple herbicide resistance Amaranthus hybridus.
Is part ofScientific Reports, 2019, vol. 10, p. 17681
European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) García, María J. et al., 2019
Showing items related by title, author, creator and subject.
Accumulation of target gene mutations confers multiple resistance to ALS, ACCase and EPSPS inhibitors in Lolium species in Chile Vazquez-Garcia, José G.; Alcántara-de la Cruz, Ricardo; Palma-Bautista, Candelario; Rojano-Delgado, Antonia M.; Cruz-Hipólito, Hugo E.; Torra Farré, Joel; Barro, Francisco; Prado, Rafael de (Frontiers Media, 2020-10-28)Different Lolium species, common weeds in cereal fields and fruit orchards in Chile, were reported showing isolated resistance to the acetyl CoA carboxylase (ACCase), acetolactate synthase (ALS) and 5-enolpyruvylshikimat ...
Reduced absorption and impaired translocation endows glyphosate resistance in Amaranthus palmeri harvested in GR soybean from Argentina Palma-Bautista, Candelario; Torra Farré, Joel; García, María J.; Bracamonte, Enzo; Rojano-Delgado, Antonia M.; Alcántara-de la Cruz, Ricardo; Prado, Rafael de ((c) American Chemical Society, 2019, 2019)Amaranthus palmeri S. Watson is probably the worst glyphosate-resistant (GR) weed worldwide. The EPSPS (5-enolpyruvylshikimate-3-phosphate-synthase) gene amplification has been reported as the major target-site-resistance ...
Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards Mora, Andrés D.; Rosario, Jesús; Rojano-Delgado, Antonia M.; Palma-Bautista, Candelario; Torra Farré, Joel; Alcántara-de la Cruz, Ricardo; Prado, Rafael de (American Chemical Society, 2019-08-15)Dominican farmers have started to apply synthetic auxin herbicides (SAHs) as the main alternative to mitigate the impacts of the occurrence of glyphosate-resistant (GR) Parthenium hysterophorus populations in citrus orchards. ...