Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors

Thumbnail
View/Open
Issue date
2019-10
Author
García, María J.
Palma-Bautista, Candelario
Vázquez-García, José G.
Rojano-Delgado, Antonia M.
Osuna, Maria D.
Torra Farré, Joel
Prado, Rafael de
Suggested citation
García, María J.; Palma-Bautista, Candelario; Vázquez-García, José G.; Rojano-Delgado, Antonia M.; Osuna, Maria D.; Torra Farré, Joel; Prado, Rafael de; . (2019) . Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors. Scientific Reports, 2019, vol. 10, p. 17681. https://doi.org/10.1038/s41598-020-74430-0.
Impact

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Amaranthus hybridus is one of the main weed species in Córdoba, Argentina. Until recently, this weed was effectively controlled with recurrent use of glyphosate. However, a population exhibiting multiple resistance (MR2) to glyphosate and imazamox appeared in a glyphosate resistant (GR) soybean field, with levels of resistance up to 93 and 38-fold higher to glyphosate and imazamox, respectively compared to the susceptible (S) population. In addition to imidazolinones, MR2 plants showed high resistance levels to sulfonylamino-carbonyl (thio) benzoates and moderate resistance to sulfonylureas and triazolopyrimidines. Multiple amino acid substitutions were found in both target genes, acetolactate synthase (ALS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), responsible for conferring high herbicides resistance levels in this A. hybridus population. In the case of EPSPS, the triple amino acid substitution TAP-IVS was found. In addition, MR2 plants also showed increased EPSPS gene expression compared to susceptible plants. A Ser653Asn substitution was found in the ALS sequence of MR2, explaining the pattern of cross-resistance to the ALS-inhibitor herbicide families found at the ALS enzyme activity level. No other mutations were found in other conserved domains of the ALS gene. This is the first report worldwide of the target site resistance mechanisms to glyphosate and ALS inhibitors in multiple herbicide resistance Amaranthus hybridus.
URI
http://hdl.handle.net/10459.1/69713
DOI
https://doi.org/10.1038/s41598-020-74430-0
Is part of
Scientific Reports, 2019, vol. 10, p. 17681
Collections

The following license files are associated with this item:

cc-by (c) García, María J. et al., 2019
Except where otherwise noted, this item's license is described as cc-by (c) García, María J. et al., 2019