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dc.contributor.authorPatrocchi-Rilo, Máximo
dc.contributor.authorGutiérrez-Martín, César-B.
dc.contributor.authorPérez-Fernández, Esther
dc.contributor.authorVilaró, Anna
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorMartínez, Sonia
dc.date.accessioned2020-10-15T12:52:04Z
dc.date.available2020-10-15T12:52:04Z
dc.date.issued2020
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10459.1/69651
dc.description.abstractForty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.
dc.description.sponsorshipJunta de Castilla y León (Consejería de Agricultura y Ganadería, Junta de Castilla y León, Spain) and Cost Action CA18217 (European Network for Optimization of Veterinary Antimicrobial Treatment).
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3390/antibiotics9090614
dc.relation.ispartofAntibiotics-Basel, 2020, vol. 9, num. 9, p. 614
dc.rightscc-by (c) Patrocchi-Rilo, Máximo et al., 2020
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPasteurella multocida
dc.subjectAntimicrobial resistance genes
dc.subjectAntimicrobial susceptibility patterns
dc.subjectSwine
dc.titleAntimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2020-10-15T12:52:04Z
dc.identifier.idgrec030449
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.3390/antibiotics9090614


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cc-by (c) Patrocchi-Rilo, Máximo et al., 2020
Except where otherwise noted, this item's license is described as cc-by (c) Patrocchi-Rilo, Máximo et al., 2020