Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
MetadataShow full item record
Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.
Is part ofAntibiotics-Basel, 2020, vol. 9, num. 9, p. 614
European research projects
Except where otherwise noted, this item's license is described as cc-by (c) Patrocchi-Rilo, Máximo et al., 2020
Showing items related by title, author, creator and subject.
Vilaró, Anna; Novell, Elena; Tarancón, Vicens; Balielles, Jordi; Vilalta, Carles; Martínez, Sonia; Fraile Sauce, Lorenzo José (MDPI, 2020)The monitoring of antimicrobial susceptibility of pig pathogens is critical to optimize antimicrobial treatments and prevent development of resistance with a one-health approach. The aim of this study was to investigate ...
Vilaró, Anna; Novell, Elena; Tarancón, Vicens; Balielles, Jordi; Allué, Eduard; Fraile Sauce, Lorenzo José (MDPI, 2020)The aim of this study was to set up antimicrobial stewardship for swine respiratory pathogens following the recommendation from the European Medicine Agency. The obtained antimicrobial susceptibility pattern recommended ...
Differential viral-host immune interactions associated with Oseltamivir-resistant H275Y and wild type H1N1 A(pdm09) Influenza virus pathogenicity Vidaña, Beatriz; Martinez-Orellana, Pamela; Martorell, Jaume; Baratelli, Massimiliano; Martínez, Jorge; García-Migura, Lourdes; Cordoba, Lorena; Pérez, Mónica; Casas, Inmaculada; Pozo, Francisco; Fraile Sauce, Lorenzo José; Majó, Natalia; Montoya, Maria (MDPI, 2020)Oseltamivir is a common therapy against influenza A virus (IAV) infections. The acquisition of oseltamivir resistance (OR) mutations, such as H275Y, hampers viral fitness. However, OR H1N1 viruses have demonstrated the ...