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dc.contributor.authorCasto-Rebollo, Cristina
dc.contributor.authorArgente, María José
dc.contributor.authorGarcía, María Luz
dc.contributor.authorPena i Subirà, Ramona Natacha
dc.contributor.authorIbáñez-Escriche, N.
dc.date.accessioned2020-09-14T10:10:14Z
dc.date.available2020-09-14T10:10:14Z
dc.date.issued2020-05-06
dc.identifier.issn0999-193X
dc.identifier.urihttp://hdl.handle.net/10459.1/69481
dc.description.abstractBackground: Environmental variance (VE) is partly under genetic control and has recently been proposed as a measure of resilience. Unravelling the genetic background of the VE of complex traits could help to improve resilience of livestock and stabilize their production across farming systems. The objective of this study was to identify genes and functional mutations associated with variation in VE of litter size (LS) in rabbits. To achieve this, we combined the results of a genome-wide association study (GWAS) and a whole-genome sequencing (WGS) analysis using data from two divergently selected rabbit lines for high and low VE of LS. These lines differ in terms of biomarkers of immune response and mortality. Moreover, rabbits with a lower VE of LS were found to be more resilient to infections than animals with a higher VE of LS. Results: By using two GWAS approaches (single-marker regression and Bayesian multiple-marker regression), we identified four genomic regions associated with VE of LS, on chromosomes 3, 7, 10, and 14. We detected 38 genes in the associated genomic regions and, using WGS, we identified 129 variants in the splicing, UTR, and coding (missense and frameshift effects) regions of 16 of these 38 genes. These genes were related to the immune system, the development of sensory structures, and stress responses. All of these variants (except one) segregated in one of the rabbit lines and were absent (n = 91) or fixed in the other one (n = 37). The fixed variants were in the HDAC9, ITGB8, MIS18A, ENSOCUG00000021276 and URB1 genes. We also identified a 1-bp deletion in the 3'UTR region of the HUNK gene that was fixed in the low VE line and absent in the high VE line. Conclusions: This is the first study that combines GWAS and WGS analyses to study the genetic basis of VE. The new candidate genes and functional mutations identified in this study suggest that the VE of LS is under the control of functions related to the immune system, stress response, and the nervous system. These findings could also explain differences in resilience between rabbits with homogeneous and heterogeneous VE of litter size.
dc.description.sponsorshipThis study was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) with the Projects AGL2014-55921, C2-1-P and C2-2-P, and AGL2017-86083, C2-1-P and C2-2-P and the Grant RYC-2016-19764.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherBMC
dc.relationMINECO/PN2013-2016/AGL2014‐55921‐C2‐1‐P
dc.relationMINECO/PN2013-2016/AGL2014‐55921‐C2‐2‐P
dc.relationMINECO/PN2017-2020/AGL2017-86083-C2-1-P
dc.relationMINECO/PN2017-2020/AGL2017-86083-C2-2-P
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1186/s12711-020-00542-w
dc.relation.ispartofGenetics Selection Evolution, 2020, vol. 52, num. 2, p. 22
dc.rightscc-by (c) Casto-Rebollo, Cristina et al., 2020
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleIdentification of functional mutations associated with environmental variance of litter size in rabbits
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2020-09-14T10:10:14Z
dc.identifier.idgrec030426
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1186/s12711-020-00542-w


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cc-by (c) Casto-Rebollo, Cristina et al., 2020
Except where otherwise noted, this item's license is described as cc-by (c) Casto-Rebollo, Cristina et al., 2020