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dc.contributor.authorRossell, Joana
dc.contributor.authorBrindefalk, Björn
dc.contributor.authorBaena-Fustegueras, Juan A
dc.contributor.authorPeinado-Onsurbe, Julia
dc.contributor.authorUdekwu, Klas I.
dc.date.accessioned2020-03-20T11:05:57Z
dc.date.available2020-03-20T11:05:57Z
dc.date.issued2020
dc.identifier.issn1436-6207
dc.identifier.urihttp://hdl.handle.net/10459.1/68279
dc.description.abstractPurpose Obesity, a worldwide health problem, is linked to an abnormal gut microbiota and is currently most efectively treated by bariatric surgery. Our aim was to characterize the microbiota of high-fat fed Sprague–Dawley rats when subjected to bariatric surgery (i.e., vertical sleeve gastrectomy) and posterior refeeding with either a high-fat or control diet. We hypothesized that bariatric surgery followed by the control diet was more efective in reverting the microbiota modifcations caused by the high-fat diet when compared to either of the two factors alone. Methods Using next-generation sequencing of ribosomal RNA amplicons, we analyzed and compared the composition of the cecal microbiota after vertical sleeve gastrectomy with control groups representing non-operated rats, control fed, high-fat fed, and post-operative diet-switched animals. Rats were fed either a high-fat or control low-fat diet and were separated into three comparison groups after eight weeks comprising no surgery, sham surgery, and vertical sleeve gastrectomy. Half of the rats were then moved from the HFD to the control diet. Using next-generation sequencing of ribosomal RNA amplicons, we analyzed the composition of the cecal microbiota of rats allocated to the vertical sleeve gastrectomy group and compared it to that of the non-surgical, control fed, high-fat fed, and post-operative diet-switched groups. Additionally, we correlated diferent biological parameters with the genera exhibiting the highest variation in abundance between the groups. Results The high-fat diet was the strongest driver of altered taxonomic composition, relative microbial abundance, and diversity in the cecum. These efects were partially reversed in the diet-switched cohort, especially when combined with sleeve gastrectomy, resulting in increased diversity and shifting relative abundances. Several highly-afected genera were correlated with obesity-related parameters. Conclusions The dysbiotic state caused by high-fat diet was improved by the change to the lower fat, higher fber control diet. Bariatric surgery contributed signifcantly and additively to the diet in restoring microbiome diversity and complexity. These results highlight the importance of dietary intervention following bariatric surgery for improved restoration of cecal diversity, as neither surgery nor change of diet alone had the same efects as when combined.ca_ES
dc.description.sponsorshipThis work was supported by the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III (ISCIII) (PI11/01159, PI15/00190 and PI15/00332 to JAB-F and JP-O), was co-fnanced by European Union Funds, Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa”. Additional funding from the Stockholm Health Authority grant # SLL 20160933 was awarded to KIUca_ES
dc.language.isoengca_ES
dc.publisherSpringerca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1007/s00394-020-02190-8ca_ES
dc.relation.ispartofEuropean Journal of Nutrition, 2020ca_ES
dc.rightscc-by (c) Rosell et al., 2020ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBariatric surgeryca_ES
dc.subjectHigh-fat dietca_ES
dc.subjectRat modelsca_ES
dc.subjectMicrobiotaca_ES
dc.titleDiet change afects intestinal microbiota restoration and improves vertical sleeve gastrectomy outcome in diet-induced obese ratsca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s00394-020-02190-8


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cc-by (c) Rosell et al., 2020
Except where otherwise noted, this item's license is described as cc-by (c) Rosell et al., 2020