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dc.contributor.authorGonzalo Benito, Hugo
dc.contributor.authorNogueras Penabad, Lara
dc.contributor.authorGil-Sánchez, Anna
dc.contributor.authorVicente Hervás, José
dc.contributor.authorValcheva, Petya
dc.contributor.authorGonzález Mingot, Cristina
dc.contributor.authorMartin-Gari, Meritxell
dc.contributor.authorCanudes, Marc
dc.contributor.authorPeralta Moncusí, Silvia
dc.contributor.authorSolana Moga, M. José
dc.contributor.authorPamplona Gras, Reinald
dc.contributor.authorPortero Otín, Manuel
dc.contributor.authorBoada Pallàs, Jordi
dc.contributor.authorSerrano Casasola, José Carlos Enrique
dc.contributor.authorBrieva Ruiz, Luis
dc.identifier.issn1662-4548 (Print)
dc.identifier.issn1662-453X (Online)
dc.description.abstractLiterature suggests that oxidative stress (OS) may be involved in the pathogenesis of multiple sclerosis (MS), in which the immune system is known to play a key role. However, to date, the OS in peripheral lymphocytes and its contribution to the disease remain unknown. The aim of the present study was to explore the influence of OS in peripheral lymphocytes of MS patients. To that end, a cross-sectional, observational pilot study was conducted [n = 58: 34 MS and 24 healthy subjects (control group)]. We have measured superoxide production and protein mitochondrial complex levels in peripheral blood mononuclear cells (PBMCs) isolated from MS patients and control. Lactate levels and the antioxidant capacity were determined in plasma. We adjusted the comparisons between study groups by age, sex and cell count according to case. Results demonstrated that PBMCs, specifically T cells, from MS patients exhibited significantly increased superoxide anion production compared to control group (p = 0.027 and p = 0.041, respectively). Increased superoxide production in PBMCs was maintained after the adjustment (p = 0.044). Regarding mitochondrial proteins, we observe a significant decrease in the representative protein content of the mitochondrial respiratory chain complexes I-V in PBMCs of MS patients (p = 0.002, p = 0.037, p = 0.03, p = 0.044, and p = 0.051, respectively), which was maintained for complexes I, III, and V after the adjustment (p = 0.026; p = 0.033; p = 0.033, respectively). In MS patients, a trend toward increased plasma lactate concentration was detected [8.04 mg lactate/dL (5.25, 9.49) in the control group, 11.36 mg lactate/dL (5.41, 14.81) in MS patients] that was statistically significant after the adjustment (p = 0.013). This might be indicative of compromised mitochondrial function. Finally, antioxidant capacity was also decreased in plasma from MS patients, both before (p = 0.027) and after adjusting for sex and age (p = 0.006). Our findings demonstrate that PBMCs of MS patients show impaired mitochondrial redox status and deficient antioxidant capacity. These results demonstrate for the first time the existence of mitochondrial alterations in the cells immune cells of MS patients already at the peripheral level.ca_ES
dc.description.sponsorshipWe acknowledge funding from the Biogen Idec Laboratories (ES-TYS-13-10491), the Spanish Ministry of Economy and Competitiveness (PI-1400328), the Autonomous Catalonia Government (ref. 20145GR1681), the Spanish Network of Multiple Sclerosis (REEM) managed by the Carlos III Health Institute (ISCIII) exp. RD16/0015/0008, and FEDER funds from the European Union (“a way to build Europe”).ca_ES
dc.publisherFrontiers Mediaca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofFrontiers in Neuroscience, 2019, vol. 13ca_ES
dc.rightscc-by, (c) Gonzalo et al., 2019ca_ES
dc.subjectMultiple sclerosisca_ES
dc.subjectOxidative stressca_ES
dc.subjectSuperoxide anionca_ES
dc.subjectMitochondrial complexesca_ES
dc.titleImpairment of Mitochondrial Redox Status in Peripheral Lymphocytes of Multiple Sclerosis Patientsca_ES

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